Analytical services
From plasmid stability studies and capillary electrophoresis to genotyping, quality control, and full-length sequencing, we deliver reliable results to support the safety and success of your research, development, and production projects.
Plasmid stability studies
Early assessment of your plasmid helps anticipate variations that could compromise your project. It includes analyzing the behavior of plasmid DNA during bacterial culture, particularly the stability of the supercoiled form and the poly(A) sequence.
Why test stability ?
Early assessment is the best way to anticipate, at every stage, variations that could affect the success of your project:
- Plasmid backbone development: assessing the stability of new plasmid constructs.
- Scale-up preparation: ensuring quality when transitioning to large-scale production.
- Process optimization: predicting and minimizing unwanted recombination depending on the strains or media used.
- Développement du backbone plasmidique : Évaluation de la stabilité de nouvelles constructions de plasmides.
- Préparation au scale-up : Garantir la qualité lors du passage à la production à grande échelle.
- Optimisation des procédés : Prédire et minimiser les recombinaisons indésirables selon les souches ou milieux utilisés.
The RD-Biotech advantage
- Extensive, well-established expertise in molecular biology and plasmid analysis, with a particular focus on poly(A) tail behavior and supercoiling levels.
- Reliable methods that deliver reproducible results for rigorous monitoring of your constructs.
- Data-driven results that directly improve and safeguard your production processes.
- Flexible services tailored to the specific needs of academic, biotechnology, and pharmaceutical projects.
Complete plasmid sequencing
Do you have a plasmid from another laboratory and need to confirm its full sequence before adding it to your database and validating its functionality?
RD-Biotech can perform this analysis for you.
Capillary gel electrophoresis
Capillary gel electrophoresis (CGE) is a precise analytical method for plasmid DNA quality control. Its high resolution enables clear differentiation between the various plasmid topologies and forms.
Genotyping by PCR
We provide PCR genotyping expertise to detect specific DNA sequences in your biological samples. Based on the targeted amplification of genomic regions of interest, this approach enables precise and reliable identification of genetic markers, mutations, insertions, and deletions, as well as transgene verification.
This service includes the extraction of target gDNA from your sample (blood, tissue, bacteria, cells, plants, etc.), the design of suitable primers, PCR performance, and verification via electrophoresis and/or sequencing.
Quality control
As part of its plasmid production activities, RD-Biotech offers a comprehensive range of quality-control analyses for both its own products and those of its clients.
Our services cover different levels of characterization and compliance to meet the specific requirements of each project, from research through regulatory-grade production.
Many analyses are performed in accordance with European Pharmacopoeia (Ph. Eur.) methods and standards, ensuring reliable results that meet current quality requirements.