{"id":90492,"date":"2021-03-25T15:19:07","date_gmt":"2021-03-25T14:19:07","guid":{"rendered":"http:\/\/www.rd-biotech.com\/proteines-recombinantes\/"},"modified":"2025-04-24T10:01:12","modified_gmt":"2025-04-24T08:01:12","slug":"recombinant-proteins","status":"publish","type":"page","link":"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/","title":{"rendered":"Recombinant proteins"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row unlock_row=&#8221;&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; style=&#8221;light&#8221; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;-4&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; shadow=&#8221;std&#8221; width=&#8221;1\/1&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;2&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; limit_content=&#8221;&#8221; uncode_shortcode_id=&#8221;465494&#8243;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;6&#8243; mobile_width=&#8221;7&#8243; width=&#8221;5\/6&#8243; uncode_shortcode_id=&#8221;196248&#8243;][vc_custom_heading heading_semantic=&#8221;p&#8221; text_size=&#8221;h4&#8243; uncode_shortcode_id=&#8221;173221&#8243; text_color_type=&#8221;uncode-solid&#8221; text_color_solid=&#8221;#a7dd1e&#8221;]<span style=\"font-weight: 400;\">RD-Biotech offers a large range of tailor-made services on its recombinant protein platform.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">By contacting RD-Biotech you will:<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400;\">Save time while devoting yourself to your major axes of high added value research<\/span><\/li>\n<li>Benefit from our scientific and technical expertise in biochemistry and recombinant proteins<\/li>\n<li>Choose the best expression system for your protein and your applications<\/li>\n<\/ul>\n<p>[\/vc_custom_heading][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;2&#8243; mobile_width=&#8221;7&#8243; width=&#8221;1\/6&#8243; uncode_shortcode_id=&#8221;194333&#8243;][vc_single_image media=&#8221;99569&#8243; media_width_percent=&#8221;80&#8243; alignment=&#8221;center&#8221; uncode_shortcode_id=&#8221;106345&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;4&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243;][vc_column_text css_animation=&#8221;bottom-t-top&#8221; uncode_shortcode_id=&#8221;100627&#8243;]RD-Biotech offers standard services but also tailor-made strategies adapted to your specifications.<\/p>\n<p>Our experts are at your disposal in order to define the best strategies, to optimize the development of your project, in terms of time and cost.[\/vc_column_text][vc_custom_heading text_color=&#8221;color-109923&#8243; heading_semantic=&#8221;h4&#8243; text_size=&#8221;h3&#8243; text_weight=&#8221;500&#8243; css_animation=&#8221;bottom-t-top&#8221; uncode_shortcode_id=&#8221;730523&#8243; text_color_type=&#8221;uncode-palette&#8221;]Recombinant protein expression platform:[\/vc_custom_heading][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;140755&#8243; css=&#8221;.vc_custom_1705308889411{margin-bottom: 100px !important;}&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;144110&#8243;][vc_custom_heading heading_semantic=&#8221;h4&#8243; text_size=&#8221;h3&#8243; text_weight=&#8221;600&#8243; css_animation=&#8221;bottom-t-top&#8221; uncode_shortcode_id=&#8221;130604&#8243;]A MULTI SYSTEM OFFER[\/vc_custom_heading][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; display=&#8221;inline&#8221; top_margin=&#8221;yes&#8221; css_animation=&#8221;bottom-t-top&#8221; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fwp-content%2Fuploads%2F2024%2F01%2FRD-Biotech-recombinant-proteins-a-multisystem-offer-VE.jpg|target:_blank&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;323540&#8243;]DISCOVER THE MULTI-SYSTEM OFFER[\/vc_button][\/vc_column_inner][\/vc_row_inner][vc_row_inner limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/3&#8243;][vc_icon icon=&#8221;fa fa-text-align-left&#8221; icon_image=&#8221;88877&#8243; icon_color=&#8221;color-193705&#8243; media_size=&#8221;50&#8243; heading_semantic=&#8221;h4&#8243; title=&#8221;Expert service and in silico feasibility study&#8221; uncode_shortcode_id=&#8221;370563&#8243; icon_color_type=&#8221;uncode-palette&#8221;]Analysis of sequence configuration, protein structure, predisposition analysis of protein expression, structural biology (Spectrometry, Dichroism, Biacore &#8230;)[\/vc_icon][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; width=&#8221;1\/3&#8243;][vc_icon icon_image=&#8221;88879&#8243; icon_color=&#8221;color-193705&#8243; media_size=&#8221;50&#8243; heading_semantic=&#8221;h4&#8243; title=&#8221;Construction of the expression vector&#8221; uncode_shortcode_id=&#8221;582168&#8243; icon_color_type=&#8221;uncode-palette&#8221;]<span style=\"font-weight: 400;\">In case you do not have the expression vector, we offer the design and cloning of the suitable and optimized vector for the desired expression system.<\/span>[\/vc_icon][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/3&#8243;][vc_icon icon_image=&#8221;88883&#8243; icon_color=&#8221;color-193705&#8243; media_size=&#8221;50&#8243; heading_semantic=&#8221;h4&#8243; title=&#8221;Feasibility study&#8221; uncode_shortcode_id=&#8221;702805&#8243; icon_color_type=&#8221;uncode-palette&#8221;]Pilot expression and purification, tests for different expression conditions (evaluation of different strains or cell lines in parallel, tests for different culture media, temperature conditions, etc.): determination of optimal performance conditions and expression performance, analysis of the results and report.<\/p>\n<p><em>Customer GO \/ NO GO for large scale productio<\/em>[\/vc_icon][\/vc_column_inner][\/vc_row_inner][vc_row_inner limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/3&#8243;][vc_icon icon_image=&#8221;88881&#8243; icon_color=&#8221;color-193705&#8243; media_size=&#8221;50&#8243; heading_semantic=&#8221;h4&#8243; title=&#8221;Production and purification of the desired quantity&#8221; uncode_shortcode_id=&#8221;172035&#8243; icon_color_type=&#8221;uncode-palette&#8221;][\/vc_icon][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; width=&#8221;1\/3&#8243;][vc_icon icon_image=&#8221;88873&#8243; icon_color=&#8221;color-193705&#8243; media_size=&#8221;50&#8243; heading_semantic=&#8221;h4&#8243; title=&#8221;Quality controls&#8221; uncode_shortcode_id=&#8221;120758&#8243; icon_color_type=&#8221;uncode-palette&#8221;]<\/p>\n<ul>\n<li aria-level=\"1\">Standard controls: SDS PAGE, agarose gel electrophoresis, spectrophotometry<\/li>\n<li aria-level=\"1\">Additional controls according to request: ELISA, Western blotting, Cytometry, Endotoxins, Host Cell Proteins, activity tests &#8230;<\/li>\n<\/ul>\n<p>[\/vc_icon][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/3&#8243;][vc_icon icon_image=&#8221;88875&#8243; icon_color=&#8221;color-193705&#8243; media_size=&#8221;50&#8243; heading_semantic=&#8221;h4&#8243; title=&#8221;Supplying of the batch of purified protein&#8221; uncode_shortcode_id=&#8221;998305&#8243; icon_color_type=&#8221;uncode-palette&#8221;]accompanied by the production \/ purification report and quality controls (buffer, concentration, aliquoting)[\/vc_icon][\/vc_column_inner][\/vc_row_inner][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fwp-content%2Fuploads%2F2024%2F01%2FRecombinant-protein-service-Eng.jpg|target:_blank&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;848891&#8243;]DISCOVER THE RECOMBINANT PROTEIN EXPRESSION PLATFORM[\/vc_button][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;4&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-193705&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; shadow=&#8221;lg&#8221; shadow_darker=&#8221;yes&#8221; width=&#8221;1\/1&#8243;][vc_column_text uncode_shortcode_id=&#8221;202732&#8243;]THE RD-BIOTECH QUALITY<\/p>\n<ul>\n<li style=\"text-align: left;\"><span style=\"font-weight: 400;\">Optimized production systems adapted to your applications: bacterial, mammalian, yeast and cell-free systems.<\/span><\/li>\n<li style=\"text-align: left;\"><strong>\u201cMulti-system expression\u201d<\/strong>\u00a0offer: Possibility to test different expression systems in parallel.<\/li>\n<li style=\"text-align: left;\"><strong>Molecular biology<\/strong>\u00a0platform for a complete service, from the construction of the expression vector to the supply of the purified and controlled protein<\/li>\n<li style=\"text-align: left;\">R&amp;D\u00a0<strong>quality<\/strong>\u00a0and \u201cendofree\u201d quality<\/li>\n<li style=\"text-align: left;\"><strong>Capacity<\/strong>: production of pilot batches and large-scale production (up to several hundred mg depending on the system)<\/li>\n<li style=\"text-align: left;\"><strong>Speed<\/strong>: Supply of purified and controlled batches in less than 3 weeks.<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;2&#8243; back_color=&#8221;color-lxmt&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; animation_speed=&#8221;1000&#8243; width=&#8221;1\/1&#8243;]\n\t\t<div id=\"tdc_summary_253449\" class=\"tdc_summary text-lead wpb_content_element \">\n\n\t\t\t<div class=\"tdc_summary_title\">\n\t\t\t\t<a href=\"#\" class=\"summaryAnchor\">Summary<i class=\"fa fa-chevron-down close\" aria-hidden=\"true\"><\/i><\/a>\n\t\t\t<\/div>\n\n\t\t<\/div>\n\t\t[\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column width=&#8221;1\/3&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/3&#8243;][vc_custom_heading text_size=&#8221;h1&#8243; text_weight=&#8221;200&#8243; el_class=&#8221;picto_after_right&#8221; uncode_shortcode_id=&#8221;188401&#8243;]Bioproduction[\/vc_custom_heading][\/vc_column][vc_column width=&#8221;1\/3&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; auto_height_device=&#8221;yes&#8221; uncode_shortcode_id=&#8221;101756&#8243; row_name=&#8221;Expression of recombinant proteins in E. coli system&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; back_color=&#8221;color-lxmt&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;7\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; text_weight=&#8221;700&#8243; uncode_shortcode_id=&#8221;199408&#8243;]Expression of recombinant proteins in\u00a0<em>E. coli<\/em>\u00a0system[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;793429&#8243;]<\/p>\n<ul>\n<li>An expertise of more than 20 years in bacteriology, with the expert support of the mAbexperts group<\/li>\n<li>This prokaryotic system offers the best compromise between expression yields and production costs.<\/li>\n<li>A set of tests, complementary to the standard process, to optimize the yield and quality of the proteins produced and purified<\/li>\n<\/ul>\n<p><b>Scale-up production capacity:<\/b>\u00a0from a few mg to several hundred mg[\/vc_column_text][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Step 1 (optional) : construction of the expression vector&#8221; tab_id=&#8221;d21d5937-3bb0-21617960985185162376787651716238331504211623833317545162383366908816238337929571623834953392&#8243; slug=&#8221;construction-vecteur-ecoli&#8221;][vc_column_text uncode_shortcode_id=&#8221;707541&#8243;]<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\">(the vector can also be provided by the client)\n<ul>\n<li>Synthesis of the gene of interest (if necessary)<\/li>\n<li>Subcloning in the expression vector. Screening<\/li>\n<li>Sequencing control<\/li>\n<li>Amplification and quality controls<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fen%2Fmolecular-biology%2F|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;168868&#8243;]DISCOVER MOLECULAR BIOLOGY[\/vc_button][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Step 2 : pilot expression in an E. coli system&#8221; tab_id=&#8221;6dad8f3c-7309-81617960985185162376787651716238331504211623833317545162383366908816238337929571623834953392&#8243; slug=&#8221;pilote-expression-ecoli&#8221;][vc_column_text uncode_shortcode_id=&#8221;174858&#8243;]<\/p>\n<ul>\n<li>Pilot study based on culture volumes of a few dozen ml<\/li>\n<li>Protein control induced by SDS-PAGE gel electrophoresis<\/li>\n<li>Western-blotting control (anti-tag antibody or specific antibody)<\/li>\n<li>Protein purification by affinity chromatography on Nickel Agarose (HIS tag) (other method possible,\u00a0<a href=\"https:\/\/web.archive.org\/web\/20230128062116\/https:\/\/www.rd-biotech.com\/en\/contact\/\">contact us<\/a>)<\/li>\n<li>Quality control: Control of the purified protein by electrophoresis on SDS-PAGE gel and protein assay<\/li>\n<\/ul>\n<p><b>If necessary:\u00a0 Additional expression tests:<\/b><\/p>\n<ul>\n<li>Transformation of different bacterial strains<\/li>\n<li>Selection of the best producers<\/li>\n<li>Tests of different expression conditions (induction type, temperature \u2026)<\/li>\n<li>Renaturation tests<\/li>\n<li>Solubilization tests<\/li>\n<\/ul>\n<p><strong><em>Go No Go<\/em><\/strong>[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Step 3 : large Scale production and purification according to a validated procedure&#8221; tab_id=&#8221;1623833716180-2-816238337929571623834953392&#8243; slug=&#8221;production-ecoli&#8221;][vc_column_text uncode_shortcode_id=&#8221;117510&#8243;]<b>Scale-up production capacity: for Research batches (batches from a few mg up to several hundred mg)<\/b><\/p>\n<ul>\n<li>Higher volume production<\/li>\n<li>Purification by affinity chromatography on Nickel Agarose (or other method)<\/li>\n<li>Control of the purified protein by electrophoresis on SDS-PAGE gel and protein assay<\/li>\n<li>Option: Elimination of the tag<\/li>\n<li>Possibility of concentration, aliquoting, lyophilization, conjugation \u2026<\/li>\n<li>Packaging and dispatch of the protein, transport in dry ice, accompanied by the report<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243;][vc_single_image media=&#8221;89056&#8243; media_width_use_pixel=&#8221;yes&#8221; alignment=&#8221;center&#8221; media_width_pixel=&#8221;300&#8243;][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243;][vc_single_image media=&#8221;89058&#8243; media_width_use_pixel=&#8221;yes&#8221; alignment=&#8221;center&#8221; media_width_pixel=&#8221;300&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_accordion_tab][\/vc_accordion][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fwp-content%2Fuploads%2F2024%2F01%2Ftableau_e_coli_en.pdf|target:_blank&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;152772&#8243;]DISCOVER E. COLI EXPRESSION SYSTEM[\/vc_button][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;88710&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;5\/12&#8243; mobile_height=&#8221;280&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; auto_height_device=&#8221;yes&#8221; uncode_shortcode_id=&#8221;147682&#8243; row_name=&#8221;Expression of recombinant proteins in mammalian cells&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;89065&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;4\/12&#8243; mobile_height=&#8221;280&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; shadow=&#8221;std&#8221; width=&#8221;8\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; text_weight=&#8221;700&#8243; el_id=&#8221;cellules-mammiferes-cho-hek293&#8243; uncode_shortcode_id=&#8221;128595&#8243;]Expression of recombinant proteins in mammalian cells: CHO, HEK293,&#8230;[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;136139&#8243;]<\/p>\n<ul>\n<li aria-level=\"1\">Broad expertise on many cell lines: adherent cells and cells in suspension<\/li>\n<li aria-level=\"1\">System allowing conservation of the native structure and post-translational modifications<\/li>\n<li aria-level=\"1\">System for simple and rapid evaluation of protein expression by transient transfection<\/li>\n<li aria-level=\"1\">Pilot study in culture flasks or plates on small culture volumes<\/li>\n<li aria-level=\"1\">Control of expressed proteins: Western-Blotting, ELISA, flow cytometry \u2026<\/li>\n<li aria-level=\"1\">Protein purification: different systems available, depending on the protein (purification by affinity chromatography, etc.)<\/li>\n<li aria-level=\"1\">Western-blotting control (anti-tag antibody or specific antibody) and SDS-PAGE gel<\/li>\n<\/ul>\n<p><b>Scale-up production capacity:<\/b><\/p>\n<ul>\n<li>from a few ml to several hundred litres of supernatant per batch<\/li>\n<li>from a few million to several billion cells per batch<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Step 1 (optional) : construction of the expression vector (the vector can also be provided by the client)&#8221; tab_id=&#8221;d21d5937-3bb0-216179609851851623767876517162383315042116238333175451623833669088162383379295716238349533921623835084089&#8243; slug=&#8221;construction-vecteur-cho&#8221;][vc_column_text uncode_shortcode_id=&#8221;531739&#8243;]<\/p>\n<ul>\n<li aria-level=\"1\">Synthesis of the nucleotide sequence of interest (if necessary)<\/li>\n<li aria-level=\"1\">Subcloning in the expression vector optimized for the cellular system<\/li>\n<li aria-level=\"1\">Sequencing control<\/li>\n<li aria-level=\"1\">Amplification and quality controls.<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fen%2Fmolecular-biology%2F|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;430711&#8243;]DISCOVER MOLECULAR BIOLOGY[\/vc_button][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Step 2 : pilot expression of the recombinant protein in a mammalian system&#8221; tab_id=&#8221;6dad8f3c-7309-816179609851851623767876517162383315042116238333175451623833669088162383379295716238349533921623835084089&#8243; slug=&#8221;pilote-expression-cho&#8221;][vc_column_text uncode_shortcode_id=&#8221;190977&#8243;]<\/p>\n<ul>\n<li>Pilot expression: Transfection, production monitoring of the recombinant protein.<\/li>\n<li>Pilot purification by affinity chromatography. (Option: purification under \u201cEndotoxin \u2013 free\u201d conditions).<\/li>\n<li>Quality controls:\n<ul>\n<li>Purity control by SDS-PAGE electrophoresis (reducing and non-reducing conditions).<\/li>\n<li>\u00a0Quantification<\/li>\n<li>Others depending on the project: Western blot, &#8230;<\/li>\n<\/ul>\n<\/li>\n<li>Packaging and dispatch of the recombinant protein accompanied by the production certificate.<\/li>\n<\/ul>\n<p><b><i>Go No Go<\/i><\/b><\/p>\n<ul>\n<li style=\"list-style-type: none;\"><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Step 3 : large Scale production and purification according to a validated procedure&#8221; tab_id=&#8221;1623833716180-2-8162383379295716238349533921623835084089&#8243; slug=&#8221;production-cho&#8221;][vc_single_image media=&#8221;89060&#8243; media_width_use_pixel=&#8221;yes&#8221; media_width_pixel=&#8221;350&#8243;][\/vc_accordion_tab][\/vc_accordion][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fwp-content%2Fuploads%2F2024%2F01%2Ftableau_mammifere_en.pdf|target:_blank&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;141335&#8243;]DISCOVER CHO CELL EXPRESSION SYSTEM[\/vc_button][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; auto_height_device=&#8221;yes&#8221; uncode_shortcode_id=&#8221;387712&#8243; row_name=&#8221;Expression of recombinant proteins in Cell Free in vitro system&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-142237&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; text_weight=&#8221;700&#8243; uncode_shortcode_id=&#8221;144889&#8243;]Expression of recombinant proteins in Cell Free\u00a0<em>in vitro<\/em>\u00a0system (WGE)[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;855285&#8243;]<b>The advantages of this system:<\/b><\/p>\n<ul>\n<li aria-level=\"1\">Cell-free process<\/li>\n<li aria-level=\"1\">Excellent alternative for the expression of proteins toxic to cells<\/li>\n<li aria-level=\"1\">Expression of soluble or membrane proteins<\/li>\n<li aria-level=\"1\">Better protein folding<\/li>\n<li aria-level=\"1\">No codon optimization step<\/li>\n<li aria-level=\"1\">Wide temperature range: 4 to 30 \u00b0 C<\/li>\n<li aria-level=\"1\">Open system: possibility of adding detergents, phospholipids, markers \u2026<\/li>\n<li aria-level=\"1\">Vector optimized for this cell-free system<\/li>\n<li aria-level=\"1\">Production capacity up to several mg<\/li>\n<\/ul>\n<p><b>Hundreds of constructions carried out each year (membrane proteins, viral proteins, membrane receptors, GPCR like proteins,\u2026)<\/b>[\/vc_column_text][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Step 1 : construction of the expression vector&#8221; tab_id=&#8221;d21d5937-3bb0-216179609851851623767876517162383315042116238333175451623833669088162383379295716238349533921623835158253&#8243; slug=&#8221;construction-vecteur-in-vitro&#8221;][vc_column_text uncode_shortcode_id=&#8221;401275&#8243;]<\/p>\n<ul>\n<li aria-level=\"1\">Synthesis of the nucleotide sequence of interest (if necessary)<\/li>\n<li aria-level=\"1\">Subcloning into the expression vector optimized for the cell free system.<\/li>\n<li aria-level=\"1\">Sequencing control<\/li>\n<li aria-level=\"1\">Amplification and quality controls.<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fen%2Fmolecular-biology%2F|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;359266&#8243;]DISCOVER MOLECULAR BIOLOGY[\/vc_button][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Step 2 : pilot expression of the recombinant protein in a Cell free system (wheat germ extract)&#8221; tab_id=&#8221;6dad8f3c-7309-816179609851851623767876517162383315042116238333175451623833669088162383379295716238349533921623835158253&#8243; slug=&#8221;pilote-expression-in-vitro&#8221;][vc_column_text uncode_shortcode_id=&#8221;194555&#8243;]<\/p>\n<ul>\n<li>\u00a0Pilot production and purification<\/li>\n<li>Control of proteins induced on SDS-PAGE gel<\/li>\n<li>Western-blotting control (anti-tag antibody or specific antibody)<\/li>\n<li>Protein dosage<\/li>\n<\/ul>\n<p><strong>If necessary: Additional expression tests:<\/strong><\/p>\n<ul>\n<li>Tests of different expression conditions<\/li>\n<li>Solubilization tests<\/li>\n<\/ul>\n<p><em><strong>Go No Go<\/strong><\/em>[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;3&#8243; column_padding=&#8221;2&#8243; title=&#8221;Step 3 : large Scale production and purification according to a validated procedure&#8221; tab_id=&#8221;1623833716180-2-8162383379295716238349533921623835158253&#8243; slug=&#8221;production-in-vitro&#8221;][vc_empty_space empty_h=&#8221;2&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; limit_content=&#8221;&#8221;][vc_column_inner width=&#8221;1\/2&#8243;][vc_single_image media=&#8221;89069&#8243; media_width_use_pixel=&#8221;yes&#8221; alignment=&#8221;center&#8221; media_width_pixel=&#8221;275&#8243;][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/2&#8243;][vc_single_image media=&#8221;89071&#8243; media_width_use_pixel=&#8221;yes&#8221; alignment=&#8221;center&#8221; media_width_pixel=&#8221;275&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_accordion_tab][\/vc_accordion][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fwp-content%2Fuploads%2F2024%2F01%2Ftableau_acellulaire_en.pdf|target:_blank&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;908494&#8243;]DISCOVER IN VITRO EXPRESSION SYSTEM[\/vc_button][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;88491&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/2&#8243; mobile_height=&#8221;280&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; auto_height_device=&#8221;yes&#8221; uncode_shortcode_id=&#8221;157769&#8243; row_name=&#8221;Expression of recombinant proteins in yeast system (P. pastoris, P. cerevisae)&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-142237&#8243; back_image=&#8221;102119&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;4\/12&#8243; uncode_shortcode_id=&#8221;119164&#8243; back_color_type=&#8221;uncode-palette&#8221;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_color=&#8221;color-lxmt&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;8\/12&#8243; mobile_height=&#8221;280&#8243; uncode_shortcode_id=&#8221;826820&#8243; back_color_type=&#8221;uncode-palette&#8221;][vc_custom_heading heading_semantic=&#8221;h3&#8243; text_weight=&#8221;700&#8243; uncode_shortcode_id=&#8221;643383&#8243;]Expression of recombinant proteins in yeast system (P. pastoris, P. cerevisae)[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;961736&#8243;]<b>Why choose yeast system:<\/b><\/p>\n<ul>\n<li><span style=\"font-weight: 400;\"> Post-translational system similar to that of mammalian cells, including glycosylation, phosphorylation, &#8230;\u00a0<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Relevant system for complex proteins\u00a0<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Secretion into the culture medium\u00a0<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> &#8220;Endofree&#8221; quality<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> High production yield\u00a0<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> A set of tests, complementary to the standard process, to optimize the yield and quality of the produced and purified proteins<\/span><\/li>\n<\/ul>\n<p><b>Scale-up production capacity:<\/b><span style=\"font-weight: 400;\"> from a few mg to several hundred mg.<\/span>[\/vc_column_text][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Step 1 (optional): construction of the expression vector&#8221; tab_id=&#8221;d21d5937-3bb0-216179609851851623767876517162383315042116238333175451623833669088162383379295716238349533921623835158253&#8243; slug=&#8221;construction-vecteur-in-vitro&#8221;][vc_column_text uncode_shortcode_id=&#8221;128218&#8243;](the vector can also be provided by the client)<\/p>\n<ul>\n<li>Synthesis of the gene of interest (if necessary)<\/li>\n<li>Subcloning in the expression vector. Screening<\/li>\n<li>Sequencing control<\/li>\n<li>Amplification and quality controls<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fen%2Fplasmid-dna%2F|title:ADN%20Plasmides|target:_blank&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;131604&#8243;]DISCOVER MOLECULAR BIOLOGY[\/vc_button][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Step 2: pilot expression in an yeast system&#8221; tab_id=&#8221;6dad8f3c-7309-816179609851851623767876517162383315042116238333175451623833669088162383379295716238349533921623835158253&#8243; slug=&#8221;pilote-expression-in-vitro&#8221;][vc_column_text uncode_shortcode_id=&#8221;430446&#8243;]<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Test of different conditions of expression (e.g. induction T\u00b0C,&#8230;).<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Protein purification by affinity chromatography on Nickel Agarose (HIS tag)\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Quality control: Control of the purified protein by electrophoresis on SDS-PAGE gel and protein assay<\/span><\/li>\n<\/ul>\n<p><b><i>Go No Go <\/i><\/b>[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;3&#8243; column_padding=&#8221;2&#8243; title=&#8221;Step 3: large Scale production and purification according to a validated procedure&#8221; tab_id=&#8221;1623833716180-2-8162383379295716238349533921623835158253&#8243; slug=&#8221;production-in-vitro&#8221;][vc_column_text uncode_shortcode_id=&#8221;104941&#8243;]<b>Scale-up production capacity: for Research batches (batches from a few mg up to several hundred mg)<\/b><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Higher volume production<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Protein purification by affinity chromatography on Nickel Agarose (HIS tag)\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Quality control: Control of the purified protein by electrophoresis on SDS-PAGE gel and protein assay<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><i><span style=\"font-weight: 400;\">Option<\/span><\/i><span style=\"font-weight: 400;\">: Elimination of the tag<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Possibility of concentration, aliquoting, lyophilization, conjugation\u2026<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Packaging and dispatch of the protein, transport in dry ice, accompanied by the report<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fwp-content%2Fuploads%2F2024%2F01%2FYeast-system-table-Ve.pdf|target:_blank&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;276437&#8243;]DISCOVER YEAST SYSTEM[\/vc_button][\/vc_column][\/vc_row][vc_row unlock_row=&#8221;&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;2&#8243; style=&#8221;light&#8221; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; shadow=&#8221;sm&#8221; width=&#8221;1\/1&#8243;][vc_custom_heading heading_semantic=&#8221;p&#8221; text_size=&#8221;h4&#8243; uncode_shortcode_id=&#8221;609350&#8243;]<\/p>\n<h4>Comparison between the 4 expression systems provided by RD-Biotech<\/h4>\n<p>[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;992922&#8243;]<span style=\"font-weight: 400;\">The table below gives a general idea of \u200b\u200bthe advantages of the three heterologous protein expression systems available from RD-Biotech. However, the performance shown may vary from protein to protein..<\/span>[\/vc_column_text][vc_single_image media=&#8221;102157&#8243; media_width_percent=&#8221;100&#8243; advanced=&#8221;yes&#8221; media_overlay_opacity=&#8221;50&#8243; media_padding=&#8221;2&#8243; lbox_skin=&#8221;white&#8221; media_link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fwp-content%2Fuploads%2F2021%2F07%2FComparaison-entre-les-3-systemes-dexpression-fournis-par-RD-Biotech-e1626797114731.png|target:_blank&#8221; uncode_shortcode_id=&#8221;720690&#8243;][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;7&#8243; top_padding=&#8221;4&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column width=&#8221;1\/4&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243;][vc_custom_heading text_size=&#8221;h1&#8243; text_weight=&#8221;200&#8243; uncode_shortcode_id=&#8221;188461&#8243;]Custom services[\/vc_custom_heading][\/vc_column][vc_column width=&#8221;1\/4&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; uncode_shortcode_id=&#8221;617657&#8243; row_name=&#8221;In silico feasibility studies&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-193705&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; text_weight=&#8221;700&#8243; uncode_shortcode_id=&#8221;725910&#8243;]In silico feasibility studies[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;213189&#8243;]<span style=\"font-weight: 400;\">Expertise service and in silico feasibility study: project support including a scientific and technical analysis of the bibliography, study of sequence configuration, analysis of the predisposition of protein expression, structural biology, in order to propose the most appropriate sequence and system.<\/span>[\/vc_column_text][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;89067&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/2&#8243; mobile_height=&#8221;280&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; uncode_shortcode_id=&#8221;209612&#8243; row_name=&#8221;Vector construction&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;89075&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243; mobile_height=&#8221;280&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-109923&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/2&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; text_weight=&#8221;700&#8243; uncode_shortcode_id=&#8221;321581&#8243;]Vector construction[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;178544&#8243;]<\/p>\n<p id=\"tw-target-text\" class=\"tw-data-text tw-text-large tw-ta\" dir=\"ltr\" data-placeholder=\"Traduction\"><span class=\"Y2IQFc\" lang=\"en\">RD-Biotech offers two alternatives: <\/span><\/p>\n<ul>\n<li class=\"tw-data-text tw-text-large tw-ta\" dir=\"ltr\" data-placeholder=\"Traduction\"><span class=\"Y2IQFc\" lang=\"en\">Using your vectors<\/span><\/li>\n<li class=\"tw-data-text tw-text-large tw-ta\" dir=\"ltr\" data-placeholder=\"Traduction\"><span class=\"Y2IQFc\" lang=\"en\">Development of your expression vectors in our optimized systems<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fen%2Fmolecular-biology%2F|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;976135&#8243;]DISCOVER MOLECULAR BIOLOGY[\/vc_button][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;5&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; shadow=&#8221;std&#8221; width=&#8221;1\/1&#8243;][vc_custom_heading text_size=&#8221;h1&#8243; text_weight=&#8221;200&#8243; uncode_shortcode_id=&#8221;192318&#8243;]Other services[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;109988&#8243;]Our sales team as well as our scientific and technical experts are at your disposal to offer you the service adapted to your request![\/vc_column_text][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fen%2Fcontact%2F|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;159134&#8243;]CONTACT US[\/vc_button][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row unlock_row=&#8221;&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; style=&#8221;light&#8221; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;-4&#8243; [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":99909,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-90492","page","type-page","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>RD-Biotech, production of recombinant proteins in CHO, HEK, E.Coli and in vitro systems<\/title>\n<meta name=\"description\" content=\"Expression and purification of recombinant proteins, CHO, HEK, E. coli, cell-free, and yeast systems. Feasibility study and vector optimization if needed.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"RD-Biotech, production of recombinant proteins in CHO, HEK, E.Coli and in vitro systems\" \/>\n<meta property=\"og:description\" content=\"Expression and purification of recombinant proteins, CHO, HEK, E. coli, cell-free, and yeast systems. Feasibility study and vector optimization if needed.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/\" \/>\n<meta property=\"og:site_name\" content=\"RD-BIOTECH\" \/>\n<meta property=\"article:modified_time\" content=\"2025-04-24T08:01:12+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.rd-biotech.com\/wp-content\/uploads\/2025\/04\/proteine-recombinante.png\" \/>\n\t<meta property=\"og:image:width\" content=\"1000\" \/>\n\t<meta property=\"og:image:height\" content=\"390\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"13 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/\",\"url\":\"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/\",\"name\":\"RD-Biotech, production of recombinant proteins in CHO, HEK, E.Coli and in vitro systems\",\"isPartOf\":{\"@id\":\"https:\/\/www.rd-biotech.com\/en\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.rd-biotech.com\/wp-content\/uploads\/2023\/06\/RD-B_2023-PROT1.jpg\",\"datePublished\":\"2021-03-25T14:19:07+00:00\",\"dateModified\":\"2025-04-24T08:01:12+00:00\",\"description\":\"Expression and purification of recombinant proteins, CHO, HEK, E. coli, cell-free, and yeast systems. Feasibility study and vector optimization if needed.\",\"breadcrumb\":{\"@id\":\"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/#primaryimage\",\"url\":\"https:\/\/www.rd-biotech.com\/wp-content\/uploads\/2023\/06\/RD-B_2023-PROT1.jpg\",\"contentUrl\":\"https:\/\/www.rd-biotech.com\/wp-content\/uploads\/2023\/06\/RD-B_2023-PROT1.jpg\",\"width\":1920,\"height\":792},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Accueil\",\"item\":\"https:\/\/www.rd-biotech.com\/en\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Recombinant proteins\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.rd-biotech.com\/en\/#website\",\"url\":\"https:\/\/www.rd-biotech.com\/en\/\",\"name\":\"RD-BIOTECH\",\"description\":\"\",\"publisher\":{\"@id\":\"https:\/\/www.rd-biotech.com\/en\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.rd-biotech.com\/en\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/www.rd-biotech.com\/en\/#organization\",\"name\":\"RD-Biotech\",\"url\":\"https:\/\/www.rd-biotech.com\/en\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.rd-biotech.com\/en\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/www.rd-biotech.com\/wp-content\/uploads\/2021\/03\/RD-BIOTECH-bleu.svg\",\"contentUrl\":\"https:\/\/www.rd-biotech.com\/wp-content\/uploads\/2021\/03\/RD-BIOTECH-bleu.svg\",\"width\":1,\"height\":1,\"caption\":\"RD-Biotech\"},\"image\":{\"@id\":\"https:\/\/www.rd-biotech.com\/en\/#\/schema\/logo\/image\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"RD-Biotech, production of recombinant proteins in CHO, HEK, E.Coli and in vitro systems","description":"Expression and purification of recombinant proteins, CHO, HEK, E. coli, cell-free, and yeast systems. Feasibility study and vector optimization if needed.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/","og_locale":"en_US","og_type":"article","og_title":"RD-Biotech, production of recombinant proteins in CHO, HEK, E.Coli and in vitro systems","og_description":"Expression and purification of recombinant proteins, CHO, HEK, E. coli, cell-free, and yeast systems. Feasibility study and vector optimization if needed.","og_url":"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/","og_site_name":"RD-BIOTECH","article_modified_time":"2025-04-24T08:01:12+00:00","og_image":[{"width":1000,"height":390,"url":"https:\/\/www.rd-biotech.com\/wp-content\/uploads\/2025\/04\/proteine-recombinante.png","type":"image\/png"}],"twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"13 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/","url":"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/","name":"RD-Biotech, production of recombinant proteins in CHO, HEK, E.Coli and in vitro systems","isPartOf":{"@id":"https:\/\/www.rd-biotech.com\/en\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/#primaryimage"},"image":{"@id":"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/#primaryimage"},"thumbnailUrl":"https:\/\/www.rd-biotech.com\/wp-content\/uploads\/2023\/06\/RD-B_2023-PROT1.jpg","datePublished":"2021-03-25T14:19:07+00:00","dateModified":"2025-04-24T08:01:12+00:00","description":"Expression and purification of recombinant proteins, CHO, HEK, E. coli, cell-free, and yeast systems. Feasibility study and vector optimization if needed.","breadcrumb":{"@id":"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/#primaryimage","url":"https:\/\/www.rd-biotech.com\/wp-content\/uploads\/2023\/06\/RD-B_2023-PROT1.jpg","contentUrl":"https:\/\/www.rd-biotech.com\/wp-content\/uploads\/2023\/06\/RD-B_2023-PROT1.jpg","width":1920,"height":792},{"@type":"BreadcrumbList","@id":"https:\/\/www.rd-biotech.com\/en\/recombinant-proteins\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Accueil","item":"https:\/\/www.rd-biotech.com\/en\/"},{"@type":"ListItem","position":2,"name":"Recombinant proteins"}]},{"@type":"WebSite","@id":"https:\/\/www.rd-biotech.com\/en\/#website","url":"https:\/\/www.rd-biotech.com\/en\/","name":"RD-BIOTECH","description":"","publisher":{"@id":"https:\/\/www.rd-biotech.com\/en\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.rd-biotech.com\/en\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/www.rd-biotech.com\/en\/#organization","name":"RD-Biotech","url":"https:\/\/www.rd-biotech.com\/en\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.rd-biotech.com\/en\/#\/schema\/logo\/image\/","url":"https:\/\/www.rd-biotech.com\/wp-content\/uploads\/2021\/03\/RD-BIOTECH-bleu.svg","contentUrl":"https:\/\/www.rd-biotech.com\/wp-content\/uploads\/2021\/03\/RD-BIOTECH-bleu.svg","width":1,"height":1,"caption":"RD-Biotech"},"image":{"@id":"https:\/\/www.rd-biotech.com\/en\/#\/schema\/logo\/image\/"}}]}},"_links":{"self":[{"href":"https:\/\/www.rd-biotech.com\/en\/wp-json\/wp\/v2\/pages\/90492","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.rd-biotech.com\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.rd-biotech.com\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.rd-biotech.com\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.rd-biotech.com\/en\/wp-json\/wp\/v2\/comments?post=90492"}],"version-history":[{"count":58,"href":"https:\/\/www.rd-biotech.com\/en\/wp-json\/wp\/v2\/pages\/90492\/revisions"}],"predecessor-version":[{"id":107702,"href":"https:\/\/www.rd-biotech.com\/en\/wp-json\/wp\/v2\/pages\/90492\/revisions\/107702"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.rd-biotech.com\/en\/wp-json\/wp\/v2\/media\/99909"}],"wp:attachment":[{"href":"https:\/\/www.rd-biotech.com\/en\/wp-json\/wp\/v2\/media?parent=90492"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}