{"id":90492,"date":"2026-03-12T15:19:07","date_gmt":"2026-03-12T14:19:07","guid":{"rendered":"https:\/\/www.rd-biotech.com\/proteines-recombinantes\/"},"modified":"2026-07-31T14:46:59","modified_gmt":"2026-07-31T12:46:59","slug":"recombinant-protein-expression","status":"publish","type":"page","link":"https:\/\/www.rd-biotech.com\/en\/recombinant-protein-expression\/","title":{"rendered":"Recombinant protein expression"},"content":{"rendered":"

[vc_row row_height_percent=”40″ back_image=”109703″ back_repeat=”no-repeat” back_position=”center center” overlay_alpha=”50″ gutter_size=”3″ column_width_percent=”100″ shift_y=”0″ z_index=”0″ content_parallax=”0″ uncode_shortcode_id=”155524″ row_height_pixel=”400″][vc_column column_width_use_pixel=”yes” position_horizontal=”left” position_vertical=”middle” gutter_size=”3″ style=”dark” overlay_alpha=”50″ shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ align_mobile=”align_center_mobile” mobile_width=”0″ width=”1\/1″ uncode_shortcode_id=”169770″ column_width_pixel=”750px”][uncode_breadcrumbs][vc_custom_heading auto_text=”yes” text_size=”fontsize-155944″ text_weight=”200″ text_space=”fontspace-781688″ uncode_shortcode_id=”497824″][\/vc_custom_heading][vc_custom_heading text_size=”h5″ text_weight=”200″ css_animation=”alpha-anim” animation_speed=”2000″ uncode_shortcode_id=”893978″]Express your protein using the optimal system\u2014CHO, HEK293, E. coli<\/em>, wheat-germ cell-free, or yeast. From in silico feasibility assessment to the delivery of purified batches within just a few weeks.[\/vc_custom_heading][vc_empty_space empty_h=”3″][vc_button button_color=”color-xsdn” radius=”btn-circle” hover_fx=”full-colored” border_width=”0″ display=”inline” inline_mobile=”yes” link=”url:%2Fcontact-rd-biotech-cro-cdmo-francaise%2F” button_color_type=”uncode-palette” uncode_shortcode_id=”706055″]Contact us[\/vc_button][vc_button button_color=”color-xsdn” radius=”btn-circle” hover_fx=”outlined” outline=”yes” border_width=”0″ display=”inline” link=”url:https%3A%2F%2Fwww.rd-biotech.com%2Fwp-content%2Fuploads%2F2021%2F08%2FRD-Biotech-unite-de-production-dADN-IndustriePharma-Juillet-2021.pdf|target:_blank” button_color_type=”uncode-palette” uncode_shortcode_id=”439894″]Download our brochure[\/vc_button][\/vc_column][\/vc_row][vc_row row_height_percent=”0″ override_padding=”yes” h_padding=”2″ top_padding=”3″ bottom_padding=”0″ overlay_alpha=”50″ gutter_size=”3″ column_width_percent=”100″ shift_y=”0″ z_index=”0″ content_parallax=”0″ uncode_shortcode_id=”345337″ el_id=”nos-services”][vc_column column_width_percent=”100″ gutter_size=”3″ overlay_alpha=”50″ shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/1″][vc_custom_heading text_color=”color-109923″ text_size=”h1″ text_weight=”400″ uncode_shortcode_id=”207848″ text_color_type=”uncode-palette”]Our recombinant protein expression solutions[\/vc_custom_heading][\/vc_column][\/vc_row][vc_row row_height_percent=”0″ override_padding=”yes” h_padding=”2″ top_padding=”3″ bottom_padding=”0″ overlay_alpha=”50″ gutter_size=”3″ column_width_percent=”100″ shift_y=”0″ z_index=”0″ content_parallax=”0″ uncode_shortcode_id=”251358″ el_class=”services”][vc_column column_width_percent=”100″ gutter_size=”3″ overlay_alpha=”50″ shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/1″][vc_tabs tab_scrolling=”yes” uncode_shortcode_id=”605913″ el_class=”tab-services mobile-tabs-to-dropdown”][vc_tab gutter_size=”2″ column_padding=”3″ title=”Multi-system approach” tab_id=”1757666499-1-61784640594702″][vc_row_inner row_inner_height_percent=”0″ overlay_alpha=”50″ gutter_size=”3″ shift_y=”0″ z_index=”0″ uncode_shortcode_id=”175162″ el_class=”services-hover vc_custom_preserve_mobile” row_height_pixel=”200″][vc_column_inner column_width_percent=”100″ align_horizontal=”align_center” gutter_size=”3″ expand_height=”yes” overlay_alpha=”50″ shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/4″ uncode_shortcode_id=”113106″][uncode_counter value=”+ 25 years” counter_color=”color-109923″ font=”font-379643″ size=”h3″ weight=”300″ height=”fontheight-179065″ uncode_shortcode_id=”165957″ counter_color_type=”uncode-palette”][vc_column_text text_lead=”small” uncode_shortcode_id=”208380″]of experience in molecular biology and protein process development[\/vc_column_text][\/vc_column_inner][vc_column_inner column_width_percent=”100″ position_horizontal=”left” gutter_size=”3″ overlay_alpha=”50″ shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/4″ uncode_shortcode_id=”133644″][uncode_counter value=”95″ prefix=”+” counter_color=”color-109923″ font=”font-379643″ size=”h3″ weight=”300″ height=”fontheight-179065″ uncode_shortcode_id=”173145″ suffix=”%” counter_color_type=”uncode-palette”][vc_column_text text_lead=”small” uncode_shortcode_id=”108012″]purity, with very low endotoxin levels (<0.1 EU\/mg)[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/4″][uncode_counter value=”4″ prefix=”<” counter_color=”color-109923″ font=”font-379643″ size=”h3″ weight=”400″ height=”fontheight-179065″ uncode_shortcode_id=”587941″ counter_color_type=”uncode-palette” suffix=”semaines”][vc_column_text text_lead=”small” uncode_shortcode_id=”202483″]to obtain your first small-scale batches of purified protein[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/4″][uncode_counter value=”From \u00b5g to g” counter_color=”color-109923″ font=”font-379643″ size=”h3″ weight=”400″ height=”fontheight-179065″ uncode_shortcode_id=”186364″ counter_color_type=”uncode-palette”][vc_column_text text_lead=”small” uncode_shortcode_id=”102347″]quantities, with optimized scale-up[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner row_inner_height_percent=”0″ overlay_alpha=”50″ gutter_size=”3″ shift_y=”0″ z_index=”0″ uncode_shortcode_id=”256557″ el_class=”services-hover vc_custom_preserve_mobile”][vc_column_inner column_width_percent=”100″ gutter_size=”3″ expand_height=”yes” overlay_alpha=”50″ shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/2″ uncode_shortcode_id=”155037″ el_class=”service-box”][vc_custom_heading text_color=”color-193705″ heading_semantic=”h3″ text_size=”h3″ uncode_shortcode_id=”169216″ text_color_type=”uncode-palette”]Protein expression in Escherichia coli<\/em>[\/vc_custom_heading][vc_column_text uncode_shortcode_id=”163387″]Rapid biomanufacturing of recombinant proteins in E. coli<\/em><\/strong>, from gene design through purification, with optimized yields for antigens, enzymes, antibody fragments, and more.[\/vc_column_text][vc_button button_color=”color-109923″ radius=”btn-circle” hover_fx=”outlined” outline=”yes” border_width=”0″ display=”inline” top_margin=”yes” button_color_type=”uncode-palette” uncode_shortcode_id=”117452″ link=”url:%2Fen%2Frecombinant-protein-expression%2Fmulti-system-approach%2F%23protein-expression-in-escherichia-coli”]Learn more[\/vc_button][\/vc_column_inner][vc_column_inner column_width_percent=”100″ gutter_size=”3″ expand_height=”yes” back_color=”color-xsdn” overlay_alpha=”50″ radius=”hg” border_color=”color-117289″ border_style=”solid” shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/2″ uncode_shortcode_id=”709352″ back_color_type=”uncode-palette” border_color_type=”uncode-palette” css=”.vc_custom_1757673407561{border-top-width: 1px !important;border-right-width: 1px !important;border-bottom-width: 1px !important;border-left-width: 1px !important;}”][vc_custom_heading text_color=”color-193705″ heading_semantic=”h3″ text_size=”h3″ uncode_shortcode_id=”197306″ text_color_type=”uncode-palette”]Protein expression in CHO and HEK cells[\/vc_custom_heading][vc_column_text uncode_shortcode_id=”267522″]<\/p>\n

Biomanufacturing of recombinant proteins and antibodies<\/strong> in mammalian cells, including CHO and HEK293<\/strong>. We support you from sequence design through production.<\/p>\n

[\/vc_column_text][vc_button button_color=”color-109923″ radius=”btn-circle” hover_fx=”outlined” outline=”yes” border_width=”0″ display=”inline” top_margin=”yes” button_color_type=”uncode-palette” uncode_shortcode_id=”171367″ link=”url:%2Fen%2Frecombinant-protein-expression%2Fmulti-system-approach%2F%23protein-expression-in-CHO-and-HEK-cells”]Learn more[\/vc_button][\/vc_column_inner][\/vc_row_inner][vc_row_inner row_inner_height_percent=”0″ overlay_alpha=”50″ gutter_size=”3″ shift_y=”0″ z_index=”0″ uncode_shortcode_id=”479862″ el_class=”.services-hover”][vc_column_inner column_width_percent=”100″ gutter_size=”3″ expand_height=”yes” back_color=”color-xsdn” overlay_alpha=”50″ radius=”hg” border_color=”color-117289″ border_style=”solid” shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/2″ uncode_shortcode_id=”942611″ back_color_type=”uncode-palette” border_color_type=”uncode-palette” css=”.vc_custom_1784229433409{border-top-width: 1px !important;border-right-width: 1px !important;border-bottom-width: 1px !important;border-left-width: 1px !important;}”][vc_custom_heading text_color=”color-193705″ heading_semantic=”h3″ text_size=”h3″ uncode_shortcode_id=”980335″ text_color_type=”uncode-palette”]Protein expression in yeast[\/vc_custom_heading][vc_column_text uncode_shortcode_id=”945518″]High-performance yeast systems\u2014including Pichia<\/em> and Saccharomyces<\/em><\/strong>\u2014for the cost-effective production of eukaryotic proteins, offering efficient secretion, post-translational modifications, and high yields.[\/vc_column_text][vc_button button_color=”color-109923″ radius=”btn-circle” hover_fx=”outlined” outline=”yes” border_width=”0″ display=”inline” top_margin=”yes” button_color_type=”uncode-palette” uncode_shortcode_id=”102473″ link=”url:%2Fen%2Frecombinant-protein-expression%2Fmulti-system-approach%2F%23protein-expression-in-yeast”]Learn more[\/vc_button][\/vc_column_inner][vc_column_inner column_width_percent=”100″ gutter_size=”3″ expand_height=”yes” back_color=”color-xsdn” overlay_alpha=”50″ radius=”hg” border_color=”color-117289″ border_style=”solid” shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/2″ uncode_shortcode_id=”709352″ back_color_type=”uncode-palette” border_color_type=”uncode-palette” css=”.vc_custom_1757673407561{border-top-width: 1px !important;border-right-width: 1px !important;border-bottom-width: 1px !important;border-left-width: 1px !important;}”][vc_custom_heading text_color=”color-193705″ heading_semantic=”h3″ text_size=”h3″ uncode_shortcode_id=”593179″ text_color_type=”uncode-palette”]Protein expression using cell-free systems[\/vc_custom_heading][vc_column_text uncode_shortcode_id=”989738″]Wheat-germ cell-free systems<\/strong> can produce toxic, unstable, or difficult-to-express proteins<\/strong> within just a few hours.[\/vc_column_text][vc_button button_color=”color-109923″ radius=”btn-circle” hover_fx=”outlined” outline=”yes” border_width=”0″ display=”inline” top_margin=”yes” button_color_type=”uncode-palette” uncode_shortcode_id=”817929″ link=”url:%2Fen%2Frecombinant-protein-expression%2Fmulti-system-approach%2F%23protein-expression-using-cell-free-systems” el_class=”clicpopup-service-humanisation”]Learn more[\/vc_button][\/vc_column_inner][\/vc_row_inner][\/vc_tab][vc_tab gutter_size=”2″ column_padding=”3″ title=”Recombinant protein biomanufacturing” tab_id=”1757666499-2-861784640594702″][vc_row_inner row_inner_height_percent=”0″ overlay_alpha=”50″ gutter_size=”3″ shift_y=”0″ z_index=”0″ uncode_shortcode_id=”175162″ el_class=”services-hover vc_custom_preserve_mobile” row_height_pixel=”200″][vc_column_inner column_width_percent=”100″ align_horizontal=”align_center” gutter_size=”3″ expand_height=”yes” overlay_alpha=”50″ shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/4″ uncode_shortcode_id=”113106″][uncode_counter value=”+ 25 years” counter_color=”color-109923″ font=”font-379643″ size=”h3″ weight=”300″ height=”fontheight-179065″ uncode_shortcode_id=”165957″ counter_color_type=”uncode-palette”][vc_column_text text_lead=”small” uncode_shortcode_id=”208380″]of experience in molecular biology and protein process development[\/vc_column_text][\/vc_column_inner][vc_column_inner column_width_percent=”100″ position_horizontal=”left” gutter_size=”3″ overlay_alpha=”50″ shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/4″ uncode_shortcode_id=”133644″][uncode_counter value=”95″ prefix=”+” counter_color=”color-109923″ font=”font-379643″ size=”h3″ weight=”300″ height=”fontheight-179065″ uncode_shortcode_id=”173145″ suffix=”%” counter_color_type=”uncode-palette”][vc_column_text text_lead=”small” uncode_shortcode_id=”108012″]purity, with very low endotoxin levels (<0.1 EU\/mg)[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/4″][uncode_counter value=”4″ prefix=”<” counter_color=”color-109923″ font=”font-379643″ size=”h3″ weight=”400″ height=”fontheight-179065″ uncode_shortcode_id=”587941″ counter_color_type=”uncode-palette” suffix=”semaines”][vc_column_text text_lead=”small” uncode_shortcode_id=”202483″]to obtain your first small-scale batches of purified protein[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/4″][uncode_counter value=”From \u00b5g to g” counter_color=”color-109923″ font=”font-379643″ size=”h3″ weight=”400″ height=”fontheight-179065″ uncode_shortcode_id=”186364″ counter_color_type=”uncode-palette”][vc_column_text text_lead=”small” uncode_shortcode_id=”102347″]quantities, with optimized scale-up[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner row_inner_height_percent=”0″ overlay_alpha=”50″ gutter_size=”3″ shift_y=”0″ z_index=”0″ uncode_shortcode_id=”256557″ el_class=”services-hover vc_custom_preserve_mobile”][vc_column_inner column_width_percent=”100″ gutter_size=”3″ expand_height=”yes” overlay_alpha=”50″ shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/2″ uncode_shortcode_id=”189925″ el_class=”service-box”][vc_custom_heading text_color=”color-193705″ heading_semantic=”h3″ text_size=”h3″ uncode_shortcode_id=”104928″ text_color_type=”uncode-palette”]In silico feasibility assessment and system selection[\/vc_custom_heading][vc_column_text uncode_shortcode_id=”149977″]A comprehensive in silico analysis to identify the optimal expression system (CHO, HEK, E. coli<\/em>, yeast, or cell-free<\/strong>) and maximize your chances of success from the outset.[\/vc_column_text][vc_button button_color=”color-109923″ radius=”btn-circle” hover_fx=”outlined” outline=”yes” border_width=”0″ display=”inline” top_margin=”yes” button_color_type=”uncode-palette” uncode_shortcode_id=”102611″ link=”url:%2Fen%2Frecombinant-protein-expression%2Frecombinant-protein-biomanufacturing%2F%23in-silico-feasibility-assessment-and-system-selection”]Learn more[\/vc_button][\/vc_column_inner][vc_column_inner column_width_percent=”100″ gutter_size=”3″ expand_height=”yes” back_color=”color-xsdn” overlay_alpha=”50″ radius=”hg” border_color=”color-117289″ border_style=”solid” shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/2″ uncode_shortcode_id=”709352″ back_color_type=”uncode-palette” border_color_type=”uncode-palette” css=”.vc_custom_1757673407561{border-top-width: 1px !important;border-right-width: 1px !important;border-bottom-width: 1px !important;border-left-width: 1px !important;}”][vc_custom_heading text_color=”color-193705″ heading_semantic=”h3″ text_size=”h3″ uncode_shortcode_id=”765628″ text_color_type=”uncode-palette”]Expression plasmid construction and optimization[\/vc_custom_heading][vc_column_text uncode_shortcode_id=”720136″]Optimized gene synthesis and construction of your expression plasmid<\/strong>, including codon optimization and regulatory elements tailored to the selected system.[\/vc_column_text][vc_button button_color=”color-109923″ radius=”btn-circle” hover_fx=”outlined” outline=”yes” border_width=”0″ display=”inline” top_margin=”yes” button_color_type=”uncode-palette” uncode_shortcode_id=”353755″ link=”url:%2Fen%2Frecombinant-protein-expression%2Frecombinant-protein-biomanufacturing%2F%23expression-plasmid-construction-and-optimization”]Learn more[\/vc_button][\/vc_column_inner][\/vc_row_inner][vc_row_inner row_inner_height_percent=”0″ overlay_alpha=”50″ gutter_size=”3″ shift_y=”0″ z_index=”0″ uncode_shortcode_id=”479862″ el_class=”.services-hover”][vc_column_inner column_width_percent=”100″ gutter_size=”3″ expand_height=”yes” back_color=”color-xsdn” overlay_alpha=”50″ radius=”hg” border_color=”color-117289″ border_style=”solid” shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/2″ uncode_shortcode_id=”178361″ back_color_type=”uncode-palette” border_color_type=”uncode-palette” css=”.vc_custom_1757673420089{border-top-width: 1px !important;border-right-width: 1px !important;border-bottom-width: 1px !important;border-left-width: 1px !important;}”][vc_custom_heading text_color=”color-193705″ heading_semantic=”h3″ text_size=”h3″ uncode_shortcode_id=”541716″ text_color_type=”uncode-palette”]Pilot study and small-scale production[\/vc_custom_heading][vc_column_text uncode_shortcode_id=”788256″]Small-scale production to validate expression, optimize experimental conditions, and characterize your protein in terms of purity<\/strong>, identity, and activity.[\/vc_column_text][vc_button button_color=”color-109923″ radius=”btn-circle” hover_fx=”outlined” outline=”yes” border_width=”0″ display=”inline” top_margin=”yes” button_color_type=”uncode-palette” uncode_shortcode_id=”101225″ link=”url:%2Fen%2Frecombinant-protein-expression%2Frecombinant-protein-biomanufacturing%2F%23pilot-study-and-small-scale-production”]Learn more[\/vc_button][\/vc_column_inner][vc_column_inner column_width_percent=”100″ gutter_size=”3″ expand_height=”yes” back_color=”color-xsdn” overlay_alpha=”50″ radius=”hg” border_color=”color-117289″ border_style=”solid” shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/2″ uncode_shortcode_id=”709352″ back_color_type=”uncode-palette” border_color_type=”uncode-palette” css=”.vc_custom_1757673407561{border-top-width: 1px !important;border-right-width: 1px !important;border-bottom-width: 1px !important;border-left-width: 1px !important;}”][vc_custom_heading text_color=”color-193705″ heading_semantic=”h3″ text_size=”h3″ uncode_shortcode_id=”755302″ text_color_type=”uncode-palette”]Large-scale production and scale-up[\/vc_custom_heading][vc_column_text uncode_shortcode_id=”162750″]<\/p>\n

Process scale-up and large-scale batch manufacturing, including optimization, high-purity purification, and comprehensive characterization. Batches are prepared for your preclinical or clinical applications.<\/p>\n

[\/vc_column_text][vc_button button_color=”color-109923″ radius=”btn-circle” hover_fx=”outlined” outline=”yes” border_width=”0″ display=”inline” top_margin=”yes” button_color_type=”uncode-palette” uncode_shortcode_id=”704209″ link=”url:%2Fen%2Frecombinant-protein-expression%2Frecombinant-protein-biomanufacturing%2F%23Large-scale-up-production-scaling-up”]Learn more[\/vc_button][\/vc_column_inner][\/vc_row_inner][\/vc_tab][vc_tab gutter_size=”2″ column_padding=”3″ title=”Process development and scale-up” tab_id=”1774855577609-3-91784640594702″][vc_row_inner row_inner_height_percent=”0″ overlay_alpha=”50″ gutter_size=”3″ shift_y=”0″ z_index=”0″ uncode_shortcode_id=”175162″ el_class=”services-hover vc_custom_preserve_mobile” row_height_pixel=”200″][vc_column_inner column_width_percent=”100″ align_horizontal=”align_center” gutter_size=”3″ expand_height=”yes” overlay_alpha=”50″ shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/4″ uncode_shortcode_id=”113106″][uncode_counter value=”+ 25 years” counter_color=”color-109923″ font=”font-379643″ size=”h3″ weight=”300″ height=”fontheight-179065″ uncode_shortcode_id=”165957″ counter_color_type=”uncode-palette”][vc_column_text text_lead=”small” uncode_shortcode_id=”208380″]of experience in molecular biology and protein process development[\/vc_column_text][\/vc_column_inner][vc_column_inner column_width_percent=”100″ position_horizontal=”left” gutter_size=”3″ overlay_alpha=”50″ shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/4″ uncode_shortcode_id=”133644″][uncode_counter value=”95″ prefix=”+” counter_color=”color-109923″ font=”font-379643″ size=”h3″ weight=”300″ height=”fontheight-179065″ uncode_shortcode_id=”173145″ suffix=”%” counter_color_type=”uncode-palette”][vc_column_text text_lead=”small” uncode_shortcode_id=”108012″]purity, with very low endotoxin levels (<0.1 EU\/mg)[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/4″][uncode_counter value=”4″ prefix=”<” counter_color=”color-109923″ font=”font-379643″ size=”h3″ weight=”400″ height=”fontheight-179065″ uncode_shortcode_id=”587941″ counter_color_type=”uncode-palette” suffix=”semaines”][vc_column_text text_lead=”small” uncode_shortcode_id=”202483″]to obtain your first small-scale batches of purified protein[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/4″][uncode_counter value=”From \u00b5g to g” counter_color=”color-109923″ font=”font-379643″ size=”h3″ weight=”400″ height=”fontheight-179065″ uncode_shortcode_id=”186364″ counter_color_type=”uncode-palette”][vc_column_text text_lead=”small” uncode_shortcode_id=”102347″]quantities, with optimized scale-up[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner row_inner_height_percent=”0″ overlay_alpha=”50″ gutter_size=”3″ shift_y=”0″ z_index=”0″ uncode_shortcode_id=”256557″ el_class=”services-hover vc_custom_preserve_mobile”][vc_column_inner column_width_percent=”100″ gutter_size=”3″ expand_height=”yes” overlay_alpha=”50″ shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/2″ uncode_shortcode_id=”102770″ el_class=”service-box”][vc_custom_heading text_color=”color-193705″ heading_semantic=”h3″ text_size=”h3″ uncode_shortcode_id=”186001″ text_color_type=”uncode-palette”]Process development[\/vc_custom_heading][vc_column_text uncode_shortcode_id=”186213″]We develop complete upstream and downstream processes tailored to your protein, including media optimization, fermentation or bioreactor parameter optimization, and purification.[\/vc_column_text][vc_button button_color=”color-109923″ radius=”btn-circle” hover_fx=”outlined” outline=”yes” border_width=”0″ display=”inline” top_margin=”yes” button_color_type=”uncode-palette” uncode_shortcode_id=”212979″ link=”url:%2Fen%2Frecombinant-protein-expression%2Fprocess-development-and-scale-up%2F%23process-development”]Learn more[\/vc_button][\/vc_column_inner][vc_column_inner column_width_percent=”100″ gutter_size=”3″ expand_height=”yes” back_color=”color-xsdn” overlay_alpha=”50″ radius=”hg” border_color=”color-117289″ border_style=”solid” shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/2″ uncode_shortcode_id=”293088″ back_color_type=”uncode-palette” border_color_type=”uncode-palette” css=”.vc_custom_1784229490657{border-top-width: 1px !important;border-right-width: 1px !important;border-bottom-width: 1px !important;border-left-width: 1px !important;}” el_class=”service-box”][vc_custom_heading text_color=”color-193705″ heading_semantic=”h3″ text_size=”h3″ uncode_shortcode_id=”162622″ text_color_type=”uncode-palette”]Scale-up and production[\/vc_custom_heading][vc_column_text uncode_shortcode_id=”120551″]Process transfer and scale-up from the laboratory to pilot or preclinical volumes of up to several dozen liters. Production is performed under controlled and documented conditions.[\/vc_column_text][vc_button button_color=”color-109923″ radius=”btn-circle” hover_fx=”outlined” outline=”yes” border_width=”0″ display=”inline” top_margin=”yes” button_color_type=”uncode-palette” uncode_shortcode_id=”363410″ link=”url:%2Fen%2Frecombinant-protein-expression%2Fprocess-development-and-scale-up%2F%23scale-up-production”]Learn more[\/vc_button][\/vc_column_inner][\/vc_row_inner][vc_row_inner row_inner_height_percent=”0″ overlay_alpha=”50″ gutter_size=”3″ shift_y=”0″ z_index=”0″ uncode_shortcode_id=”256557″ el_class=”services-hover vc_custom_preserve_mobile”][vc_column_inner column_width_percent=”100″ gutter_size=”3″ expand_height=”yes” back_color=”color-xsdn” overlay_alpha=”50″ radius=”hg” border_color=”color-117289″ border_style=”solid” shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/1″ uncode_shortcode_id=”709352″ back_color_type=”uncode-palette” border_color_type=”uncode-palette” css=”.vc_custom_1757673407561{border-top-width: 1px !important;border-right-width: 1px !important;border-bottom-width: 1px !important;border-left-width: 1px !important;}”][vc_custom_heading text_color=”color-193705″ heading_semantic=”h3″ text_size=”h3″ uncode_shortcode_id=”194860″ text_color_type=”uncode-palette”]Advanced characterization and stability[\/vc_custom_heading][vc_column_text uncode_shortcode_id=”110186″]<\/p>\n

Stability studies conducted according to ICH guidelines, purity analysis by SEC-HPLC and DSC, physicochemical characterization, and functional activity testing for your protein batches.<\/p>\n

[\/vc_column_text][vc_button button_color=”color-109923″ radius=”btn-circle” hover_fx=”outlined” outline=”yes” border_width=”0″ display=”inline” top_margin=”yes” button_color_type=”uncode-palette” uncode_shortcode_id=”154642″ link=”url:%2Fen%2Frecombinant-protein-expression%2Fprocess-development-and-scale-up%2F%23advanced-characterization-and-stability”]Learn more[\/vc_button][\/vc_column_inner][\/vc_row_inner][\/vc_tab][\/vc_tabs][\/vc_column][\/vc_row][vc_row row_height_percent=”0″ override_padding=”yes” h_padding=”2″ top_padding=”3″ bottom_padding=”3″ overlay_alpha=”50″ gutter_size=”3″ column_width_percent=”100″ shift_y=”0″ z_index=”0″ content_parallax=”0″ uncode_shortcode_id=”918008″][vc_column column_width_percent=”100″ position_horizontal=”left” gutter_size=”3″ override_padding=”yes” column_padding=”3″ style=”dark” back_color=”color-109923″ overlay_alpha=”50″ radius=”hg” shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ css_animation=”bottom-t-top” width=”1\/1″ uncode_shortcode_id=”205203″ back_color_type=”uncode-palette”][vc_custom_heading text_size=”h1″ text_weight=”400″ uncode_shortcode_id=”200387″]The assurance of quality made in France<\/strong>[\/vc_custom_heading][vc_column_text uncode_shortcode_id=”225711″]French scientific excellence supporting your research through customized, reliable, and responsive solutions.[\/vc_column_text][vc_button button_color=”color-xsdn” radius=”btn-circle” hover_fx=”full-colored” border_width=”0″ link=”url:%2Fcontact-rd-biotech-cro-cdmo-francaise%2F” button_color_type=”uncode-palette” uncode_shortcode_id=”822658″]Contact us[\/vc_button][\/vc_column][\/vc_row][vc_row row_height_percent=”0″ override_padding=”yes” h_padding=”2″ top_padding=”3″ bottom_padding=”3″ back_color=”color-193705″ overlay_alpha=”50″ gutter_size=”3″ column_width_percent=”100″ shift_y=”0″ z_index=”0″ content_parallax=”0″ uncode_shortcode_id=”510249″ back_color_type=”uncode-palette” el_id=”nos-avantages”][vc_column column_width_percent=”100″ position_horizontal=”left” gutter_size=”3″ style=”dark” overlay_alpha=”50″ radius=”hg” shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ css_animation=”bottom-t-top” width=”5\/6″ uncode_shortcode_id=”516850″][vc_custom_heading text_size=”h1″ text_weight=”400″ uncode_shortcode_id=”145977″]Why choose RD-Biotech for your recombinant proteins?[\/vc_custom_heading][vc_column_text uncode_shortcode_id=”131723″]<\/p>\n

Expertise in difficult and toxic proteins<\/strong><\/p>\n

RD-Biotech excels in the production of recombinant proteins considered particularly challenging<\/strong>, including membrane proteins, GPCRs, cytokines, and toxic or unstable enzymes.<\/p>\n

Through our command of all major expression systems (E. coli<\/em>, CHO, HEK293, yeast, and cell-free<\/strong>) we systematically identify the best strategy for obtaining a soluble, functional, and biologically active recombinant protein<\/strong>.<\/p>\n

We have successfully completed numerous projects in which other platforms had failed, particularly involving therapeutic recombinant protein<\/strong>s and antigens used in recombinant protein vaccines<\/strong>.<\/p>\n

Our multi-system approach and extensive optimization expertise make the difference in the most demanding projects.<\/p>\n

[\/vc_column_text][vc_accordion label_border=”yes” content_border=”yes” gutter_simple=”0″ no_h_padding=”yes” uncode_shortcode_id=”136263″ active_tab=”0″][vc_accordion_tab gutter_size=”2″ column_padding=”2″ title=”En savoir +” tab_id=”1776088760-1-971784640595711″][vc_column_text uncode_shortcode_id=”214455″]<\/p>\n

Flexibility and customization<\/strong><\/p>\n

Every recombinant protein production project<\/strong> is unique. At RD-Biotech, we do not apply a standardized process. Instead, we design a customized solution based on your sequence, final application\u2014research, diagnostics, or therapeutic development\u2014and time or budget constraints.<\/p>\n

Whether you require simple transient expression in HEK cells, scale-up in CHO cells, cell-free production<\/strong>, or extensive optimization of recombinant protein cloning<\/strong> and expression, our team adapts every stage to your needs.<\/p>\n

This flexibility enables you to obtain exactly the recombinant protein you require, without compromise.<\/p>\n

Rapid small- and medium-scale production<\/strong><\/p>\n

In research environments where speed is critical, RD-Biotech offers recombinant protein production in batches ranging<\/strong> from a few milligrams to several hundred milligrams, with some of the shortest lead times on the market: only a few weeks from cloning to purified protein.<\/p>\n

Unlike large CDMOs focused on GMP manufacturing<\/strong> and high-volume production, our organization is designed to respond quickly to the needs of academic laboratories, biotechnology companies, and preclinical R&D teams.<\/p>\n

You can move your projects forward faster without waiting months to receive your recombinant protein.<\/p>\n

Dedicated technical support<\/strong><\/p>\n

With RD-Biotech, you are never on your own. You benefit from a dedicated scientific contact, Head of the Recombinant Protein Platform, or a senior expert from our immunology team<\/strong>.<\/p>\n

From the initial in silico feasibility assessment through delivery of the final batch, we support you with regular technical discussions, detailed reports, and data-driven scientific recommendations.<\/p>\n

This personalized support helps ensure the success of your recombinant protein production project while saving you valuable time.<\/p>\n

[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion][\/vc_column][vc_column column_width_percent=”100″ gutter_size=”3″ overlay_alpha=”50″ shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_visibility=”yes” mobile_width=”0″ width=”1\/6″ uncode_shortcode_id=”101795″][vc_single_image media=”109243″ media_width_percent=”100″ uncode_shortcode_id=”121598″][\/vc_column][\/vc_row][vc_row row_height_percent=”0″ override_padding=”yes” h_padding=”2″ top_padding=”3″ bottom_padding=”3″ overlay_alpha=”50″ gutter_size=”3″ column_width_percent=”100″ shift_y=”0″ z_index=”0″ content_parallax=”0″ uncode_shortcode_id=”136587″ back_color_type=”uncode-solid” back_color_solid=”#f0fbff” el_class=”equipe” el_id=”notre-equipe”][vc_column column_width_percent=”100″ position_horizontal=”left” gutter_size=”3″ overlay_alpha=”50″ radius=”hg” shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ css_animation=”bottom-t-top” width=”1\/1″ uncode_shortcode_id=”433165″][vc_row_inner row_inner_height_percent=”0″ overlay_alpha=”50″ gutter_size=”3″ shift_y=”0″ z_index=”0″ uncode_shortcode_id=”765060″ css=”.vc_custom_1757919992931{padding-bottom: 100px !important;}”][vc_column_inner width=”1\/2″][vc_custom_heading text_size=”h1″ uncode_shortcode_id=”160869″]Benefit from expert<\/strong>, tailor-made support for your immunology projects<\/strong>.[\/vc_custom_heading][vc_button button_color=”color-109923″ radius=”btn-circle” hover_fx=”full-colored” border_width=”0″ uncode_shortcode_id=”185989″ button_color_type=”uncode-palette”]Schedule a meeting[\/vc_button][\/vc_column_inner][vc_column_inner column_width_percent=”100″ align_horizontal=”align_center” gutter_size=”3″ override_padding=”yes” column_padding=”3″ overlay_alpha=”50″ radius=”hg” shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/2″ uncode_shortcode_id=”192979″ back_color_type=”uncode-solid” back_color_solid=”#ddf6ff”][vc_column_text uncode_shortcode_id=”449667″]\u00abWe develop customized solutions in molecular biology, immunology, and cell engineering, with recognized expertise in recombinant antibody production. Through robust processes and a collaborative approach, we deliver highly specific biomolecules that fully meet the requirements of research and preclinical or clinical development <\/span>\u00bb[\/vc_column_text][vc_column_text text_lead=”yes” uncode_shortcode_id=”160914″ text_color_type=”uncode-solid” text_color_solid=”#023a92″]Quentin Bardin, Commercial[\/vc_column_text][vc_single_image media=”111109″ media_width_use_pixel=”yes” shape=”img-round” radius=”hg” uncode_shortcode_id=”929817″ media_width_pixel=”200″ el_class=”equipe-perso-decale-gauche”][\/vc_column_inner][\/vc_row_inner][vc_custom_heading text_size=”h1″ uncode_shortcode_id=”637279″]Meet our immunology and protein biochemistry team<\/strong>[\/vc_custom_heading][vc_row_inner row_inner_height_percent=”0″ overlay_alpha=”50″ gutter_size=”3″ shift_y=”0″ z_index=”0″ uncode_shortcode_id=”166242″ css=”.vc_custom_1757919917365{padding-top: 32px !important;}”][vc_column_inner width=”1\/3″][vc_icon position=”left” space_reduced=”yes” icon_image=”111063″ media_size=”200px” text_size=”h5″ uncode_shortcode_id=”874566″ title=”Magali”]Head of the Antibodies, Proteins and Cells Business Unit[\/vc_icon][\/vc_column_inner][vc_column_inner width=”1\/3″][vc_icon position=”left” space_reduced=”yes” icon_image=”111065″ media_size=”200px” text_size=”h5″ uncode_shortcode_id=”164225″ title=”Romain”]<\/p>\n

Manager \u2013 Recombinant Antibody and Protein Production<\/span><\/p>\n

[\/vc_icon][\/vc_column_inner][vc_column_inner column_width_percent=”100″ gutter_size=”3″ expand_height=”yes” overlay_alpha=”50″ shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/3″ uncode_shortcode_id=”160956″][vc_icon position=”left” space_reduced=”yes” icon_image=”110273″ media_size=”200px” text_size=”h5″ uncode_shortcode_id=”147672″ title=”Anthony”]Head of Vector Construction[\/vc_icon][\/vc_column_inner][\/vc_row_inner][vc_button button_color=”color-109923″ radius=”btn-circle” hover_fx=”outlined” outline=”yes” border_width=”0″ uncode_shortcode_id=”720596″ button_color_type=”uncode-palette” link=”url:%2Fa-propos-rd-biotech%2F”]Learn more[\/vc_button][\/vc_column][\/vc_row][vc_row row_height_percent=”0″ override_padding=”yes” h_padding=”2″ top_padding=”3″ bottom_padding=”3″ overlay_alpha=”50″ gutter_size=”3″ column_width_percent=”100″ shift_y=”0″ z_index=”0″ content_parallax=”0″ uncode_shortcode_id=”163907″ el_id=”notre-experience”][vc_column column_width_use_pixel=”yes” align_horizontal=”align_center” gutter_size=”3″ overlay_alpha=”50″ radius=”hg” shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ css_animation=”bottom-t-top” width=”1\/1″ uncode_shortcode_id=”443392″ column_width_pixel=”900px”][vc_custom_heading text_color=”color-109923″ text_size=”h1″ text_weight=”400″ uncode_shortcode_id=”545719″ text_color_type=”uncode-palette”]We meet our clients\u2019 requirements by providing advanced technical solutions while optimizing budgets.[\/vc_custom_heading][vc_button button_color=”color-109923″ radius=”btn-circle” outline=”yes” border_width=”0″ link=”url:%2Fcontact-rd-biotech-cro-cdmo-francaise%2F” button_color_type=”uncode-palette” uncode_shortcode_id=”158009″]Contact us[\/vc_button][\/vc_column][\/vc_row][vc_row row_height_percent=”0″ override_padding=”yes” h_padding=”2″ top_padding=”3″ bottom_padding=”3″ overlay_alpha=”50″ gutter_size=”3″ column_width_percent=”100″ shift_y=”0″ z_index=”0″ content_parallax=”0″ uncode_shortcode_id=”135491″ back_color_type=”uncode-solid” back_color_solid=”#f0fbff” el_id=”notre-expertise”][vc_column column_width_percent=”100″ position_horizontal=”left” gutter_size=”3″ overlay_alpha=”50″ radius=”hg” shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ css_animation=”bottom-t-top” width=”1\/1″ uncode_shortcode_id=”433165″][vc_custom_heading text_size=”h1″ text_weight=”400″ uncode_shortcode_id=”157637″]Our expertise :\u00a0a practical example<\/span>[\/vc_custom_heading][vc_row_inner][vc_column_inner width=”2\/3″][vc_column_text text_lead=”yes” uncode_shortcode_id=”809083″]<\/p>\n\n\n\n
Client :<\/td>\n\"\"<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

[\/vc_column_text][vc_column_text text_lead=”yes” uncode_shortcode_id=”119273″]<\/p>\n

Objective: To produce a series of recombinant antibodies<\/strong> from sequences provided by the client and validate their functional specificity for a tumor-associated membrane target.<\/p>\n

[\/vc_column_text][vc_column_text uncode_shortcode_id=”200872″]<\/p>\n

    \n
  • Antibody reformatting from scFv to human IgG1<\/strong><\/li>\n
  • Production by transient transfection<\/strong> in HEK293 cells, with an average yield of 80 mg\/L<\/li>\n
  • Protein A affinity purification, followed by SDS-PAGE and SEC-HPLC analysis<\/li>\n
  • Characterization by ELISA<\/strong>, flow cytometry, and live-cell binding assays<\/li>\n
  • Delivery of a complete report complying with the client\u2019s quality requirements<\/li>\n<\/ul>\n

    [\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/3″][vc_single_image media=”109161″ media_width_percent=”100″ shape=”img-round” radius=”hg” uncode_shortcode_id=”192338″][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row row_height_percent=”0″ override_padding=”yes” h_padding=”2″ top_padding=”3″ bottom_padding=”3″ overlay_alpha=”50″ gutter_size=”5″ column_width_percent=”100″ shift_y=”0″ z_index=”0″ content_parallax=”0″ uncode_shortcode_id=”139086″][vc_column column_width_percent=”100″ position_horizontal=”left” gutter_size=”3″ overlay_alpha=”50″ radius=”hg” shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ css_animation=”bottom-t-top” width=”1\/4″ uncode_shortcode_id=”433165″][vc_custom_heading text_size=”h1″ text_weight=”400″ uncode_shortcode_id=”204997″]FAQ[\/vc_custom_heading][vc_column_text uncode_shortcode_id=”454729″]<\/p>\n

    Do you have questions about our recombinant proteins? Here is what you need to know to get your project off to a good start.<\/span><\/p>\n

    [\/vc_column_text][vc_button button_color=”color-109923″ radius=”btn-circle” hover_fx=”full-colored” border_width=”0″ uncode_shortcode_id=”276709″ button_color_type=”uncode-palette” link=”url:%2Fcontact”]Nous contacter[\/vc_button][\/vc_column][vc_column width=”3\/4″][vc_accordion gutter_simple=”0″ uncode_shortcode_id=”196713″][vc_accordion_tab gutter_size=”2″ column_padding=”2″ title=”What is a recombinant protein?” tab_id=”1757687938-1-601784640596522″][vc_column_text uncode_shortcode_id=”945906″ css=”.vc_custom_1784642564844{padding-right: 5px !important;}”]<\/p>\n

    A recombinant protein<\/strong> is a protein produced through genetic engineering. The gene encoding the protein of interest is inserted into an expression vector and then introduced into a host system, such as a bacterium, a eukaryotic cell, or a cell-free system.<\/p>\n

    This method enables reproducible and scalable recombinant protein production<\/strong> tailored to your needs, whether for research, diagnostics, or therapeutic applications.<\/p>\n

    [\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=”2″ column_padding=”2″ title=”Which recombinant protein expression systems do you offer?” tab_id=”1757687938-2-171784640596522″][vc_column_text uncode_shortcode_id=”119856″]<\/p>\n

    We work with five expression systems in parallel:<\/p>\n

      \n
    • E. coli<\/em><\/strong><\/li>\n
    • CHO cells<\/strong><\/li>\n
    • HEK cells<\/strong><\/li>\n
    • Yeast<\/strong><\/li>\n
    • Wheat-germ cell-free systems<\/strong><\/li>\n<\/ul>\n

      This unique multi-system approach allows us to test several hosts during the pilot phase and select the most suitable system for your recombinant protein based on yield, folding, native post-translational modifications, and other requirements.<\/p>\n

      <\/h2>\n

      [\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=”2″ column_padding=”2″ title=”How long does recombinant protein production take?” tab_id=”1757688593338-2-61784640596522″][vc_column_text uncode_shortcode_id=”160593″]<\/p>\n

        \n
      • Feasibility assessment and pilot study:<\/strong> 2\u20133 weeks<\/li>\n
      • Purified small-scale batch of 1\u201350 mg:<\/strong> 4\u20136 weeks in total<\/li>\n
      • Large-scale production:<\/strong> 6\u201310 weeks, depending on the expression system<\/li>\n<\/ul>\n

        Most pilot projects deliver purified batches in under three weeks.<\/p>\n

        [\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=”2″ column_padding=”2″ title=”Do you produce recombinant proteins in E. coli?” tab_id=”1757688611273-3-61784640596522″][vc_column_text uncode_shortcode_id=”189216″]<\/p>\n

        Yes. We are particularly experienced in recombinant protein production using E. coli<\/em>,<\/strong> offering high yields of up to 500 mg\/L or more, controlled costs, and very short lead times.<\/p>\n

        This system is ideal for simple proteins, enzymes, and intracellular domains. We also offer codon optimization and periplasmic production to improve protein solubility.<\/p>\n

        [\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=”2″ column_padding=”2″ title=”What level of purity can you achieve?” tab_id=”1757688623182-4-11784640596522″][vc_column_text uncode_shortcode_id=”509499″]<\/p>\n

        Depending on the protein of interest, we can guarantee purity levels of up to 95%<\/strong> through multistep purification, combining affinity chromatography, ion-exchange chromatography, and size-exclusion chromatography.<\/p>\n

        We can also provide tag removal, endotoxin control below 0.1 EU\/\u00b5g, and endotoxin-free or High Quality grades.<\/p>\n

        [\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=”2″ column_padding=”2″ title=”Can you produce therapeutic recombinant proteins or antigens for recombinant protein vaccines?” tab_id=”1757688623182-4-101784640596522″][vc_column_text uncode_shortcode_id=”329935″]<\/p>\n

        Absolutely. We regularly produce therapeutic recombinant proteins<\/strong> and antigens for recombinant protein vaccines in R&D<\/strong>, enhanced-quality, and endotoxin-free grades.<\/p>\n

        Our mammalian expression systems (CHO and HEK<\/strong>) as well as our yeast systems are particularly well suited to preclinical and clinical applications.<\/p>\n

        [\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=”2″ column_padding=”2″ title=”How do you manage recombinant protein cloning and expression?” tab_id=”1757688623182-4-1011784640596522″][vc_column_text uncode_shortcode_id=”934554″]<\/p>\n

        We manage the entire recombinant protein cloning and expression process<\/strong>, including:<\/p>\n

          \n
        • Codon optimization for the selected expression system<\/li>\n
        • Gene synthesis or subcloning<\/li>\n
        • Addition of tags such as His, Fc, AviTag, or SUMO<\/li>\n
        • Vector construction<\/li>\n
        • Sequencing<\/li>\n<\/ul>\n

          You may also provide your own expression vector.<\/p>\n

          [\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=”2″ column_padding=”2″ title=”What if my protein is toxic, membrane-bound, or difficult to express?” tab_id=”1757688623182-4-10151784640596522″][vc_column_text uncode_shortcode_id=”195390″]<\/p>\n

          Our wheat-germ cell-free system is often the ideal solution for toxic or membrane proteins because it avoids cellular cytotoxicity.<\/p>\n

          We systematically test several expression systems in parallel to maximize your chances of success.<\/p>\n

          [\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=”2″ column_padding=”2″ title=”How can I obtain a quotation for recombinant protein production?” tab_id=”1757688623182-4-101541784640596522″][vc_column_text uncode_shortcode_id=”879300″]<\/p>\n

          Simply send us:<\/p>\n

            \n
          • Your sequence or specifications<\/li>\n
          • The required tag<\/li>\n
          • The target quantity<\/li>\n
          • The intended application<\/li>\n<\/ul>\n

            We will provide a detailed, non-binding proposal outlining the recommended strategy, timeline, and budget within 48 hours.<\/p>\n

            <\/h2>\n

            [\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=”2″ column_padding=”2″ title=”What is the difference between CHO and HEK for recombinant protein expression?” tab_id=”1757688623182-4-1015441784640596522″][vc_column_text uncode_shortcode_id=”896945″]CHO cells<\/strong> are often preferred for larger-scale production and therapeutic applications. HEK293 cells<\/strong> are ideal for rapid testing and provide glycosylation profiles that closely resemble those found in humans.[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion][\/vc_column][\/vc_row][vc_row row_height_percent=”0″ override_padding=”yes” h_padding=”2″ top_padding=”3″ bottom_padding=”3″ overlay_alpha=”50″ gutter_size=”3″ column_width_percent=”100″ shift_y=”0″ z_index=”0″ content_parallax=”0″ uncode_shortcode_id=”583145″][vc_column column_width_percent=”100″ position_vertical=”bottom” align_horizontal=”align_center” gutter_size=”0″ override_padding=”yes” column_padding=”2″ expand_height=”yes” overlay_alpha=”50″ radius=”hg” shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”5\/12″ uncode_shortcode_id=”204056″ back_color_type=”uncode-solid” back_color_solid=”#ebf6fa”][vc_row_inner][vc_column_inner column_width_percent=”100″ align_horizontal=”align_center” gutter_size=”3″ override_padding=”yes” column_padding=”3″ overlay_alpha=”50″ shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ width=”1\/1″ uncode_shortcode_id=”563544″][vc_custom_heading uncode_shortcode_id=”279334″]Frenche <\/strong><\/span><\/span>manufacturing for international<\/strong> markets[\/vc_custom_heading][\/vc_column_inner][\/vc_row_inner][vc_button button_color=”color-193705″ radius=”btn-circle” outline=”yes” border_width=”0″ link=”url:%2Fa-propos-rd-biotech%2F” button_color_type=”uncode-palette” uncode_shortcode_id=”151570″]Learn more[\/vc_button][\/vc_column][vc_column width=”7\/12″][vc_single_image media=”109261″ media_width_percent=”100″ shape=”img-round” radius=”hg” uncode_shortcode_id=”280512″][\/vc_column][\/vc_row][vc_row row_height_percent=”0″ override_padding=”yes” h_padding=”2″ top_padding=”3″ bottom_padding=”3″ overlay_alpha=”50″ gutter_size=”3″ column_width_percent=”100″ shift_y=”0″ z_index=”0″ content_parallax=”0″ uncode_shortcode_id=”733204″ el_class=”other-services” el_id=”nous-contacter”][vc_column column_width_use_pixel=”yes” align_horizontal=”align_center” gutter_size=”3″ overlay_alpha=”50″ radius=”hg” shift_x=”0″ shift_y=”0″ shift_y_down=”0″ z_index=”0″ medium_width=”0″ mobile_width=”0″ css_animation=”bottom-t-top” width=”1\/1″ uncode_shortcode_id=”157092″][vc_custom_heading text_color=”color-109923″ text_size=”h1″ text_weight=”400″ uncode_shortcode_id=”171627″ text_color_type=”uncode-palette”]Discover our other services[\/vc_custom_heading][vc_column_text uncode_shortcode_id=”142819″]RD-Biotech has been a biotechnology CRO\/CDMO<\/strong> since 2002. We provide customized molecular biology, immunology, and cell-engineering solutions for R&D and preclinical and clinical development<\/strong>, combining expertise, responsiveness, and confidentiality.[\/vc_column_text][uncode_index el_id=”index-138264″ index_type=”carousel” loop=”size:10|order_by:date|post_type:page|tax_query:94|by_id:87925,87942,87940,19884,87944|taxonomy_count:10″ carousel_lg=”3″ carousel_md=”3″ carousel_sm=”1″ gutter_size=”3″ page_items=”media|media|onpost|original,title|-,text|excerpt,icon|sm” carousel_height=”equal” carousel_interval=”3000″ carousel_navspeed=”400″ carousel_dots=”yes” carousel_dots_mobile=”yes” stage_padding=”0″ single_overlay_opacity=”1″ single_h_align=”center” single_padding=”2″ single_title_family=”font-379643″ single_title_dimension=”h3″ single_title_weight=”500″ single_text_lead=”small” single_border=”yes” uncode_shortcode_id=”872201″ single_icon=”fa fa-arrow-right2″][vc_column_text uncode_shortcode_id=”755462″]<\/p>\n

            Would you like to clarify your expectations?<\/span><\/p>\n

            [\/vc_column_text][vc_button button_color=”color-109923″ radius=”btn-circle” hover_fx=”full-colored” border_width=”0″ uncode_shortcode_id=”176159″ button_color_type=”uncode-palette” link=”url:%2Fcontact”]Contact us[\/vc_button][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

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