{"id":90486,"date":"2021-03-25T15:22:24","date_gmt":"2021-03-25T14:22:24","guid":{"rendered":"http:\/\/www.rd-biotech.com\/culture-cellulaire\/"},"modified":"2023-06-08T16:57:22","modified_gmt":"2023-06-08T14:57:22","slug":"cell-culture","status":"publish","type":"page","link":"https:\/\/www.rd-biotech.com\/en\/cell-culture\/","title":{"rendered":"Cell culture"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row unlock_row=&#8221;&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; style=&#8221;light&#8221; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;-4&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; shadow=&#8221;std&#8221; width=&#8221;1\/1&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;2&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; uncode_shortcode_id=&#8221;107504&#8243; limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;6&#8243; mobile_width=&#8221;7&#8243; width=&#8221;5\/6&#8243; uncode_shortcode_id=&#8221;115501&#8243;][vc_custom_heading heading_semantic=&#8221;p&#8221; text_size=&#8221;h4&#8243; uncode_shortcode_id=&#8221;837645&#8243; text_color_type=&#8221;uncode-solid&#8221; text_color_solid=&#8221;#ff5050&#8243;]<span style=\"font-weight: 400;\">RD-Biotech offers a complete range of custom services on its cell culture platform.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">By contacting RD-Biotech you will:<\/span><\/p>\n<ul>\n<li>save time while devoting yourself to your major axes of high added value research,<\/li>\n<li>benefit from our scientific and technical expertise in cell engineering and cell culture.<\/li>\n<\/ul>\n<p>[\/vc_custom_heading][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;2&#8243; mobile_width=&#8221;7&#8243; width=&#8221;1\/6&#8243; uncode_shortcode_id=&#8221;747647&#8243;][vc_single_image media=&#8221;99619&#8243; media_width_percent=&#8221;80&#8243; alignment=&#8221;center&#8221; uncode_shortcode_id=&#8221;181146&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; el_class=&#8221;no-padding-left-right-mobile&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-193705&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; shadow=&#8221;lg&#8221; width=&#8221;1\/2&#8243;][vc_custom_heading heading_semantic=&#8221;h4&#8243; text_size=&#8221;h4&#8243;]Why choose RD-BIOTECH?[\/vc_custom_heading][vc_column_text]<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Do you want to obtain a sufficient <strong>cell pool<\/strong> to carry out your analyses or for the <strong>expression of your recombinant proteins<\/strong>?<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Do you want to obtain a few mg of recombinant antibodies by<strong> transient transfection<\/strong>?<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Do you want to develop a <strong>stable cell line<\/strong>?<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Do you want a <strong>cell bank<\/strong>?<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">Eukaryotic or prokaryotic cells, adherent or suspended cells, primary cells, blood cells?<\/span>[\/vc_column_text][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-193705&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; shadow=&#8221;lg&#8221; width=&#8221;1\/2&#8243;][vc_custom_heading heading_semantic=&#8221;h4&#8243; text_size=&#8221;h4&#8243;]RD-Biotech can meet your demands:[\/vc_custom_heading][vc_column_text]<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">An expertise of more than 30 years in cell culture<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Perfect mastery of cell engineering processes for many cell types<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">An analytical platform for the screening, characterization and controls of your cells<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\">The supply of a personalized service adapted to your needs<\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">A quality service, at very attractive costs and very short deadlines<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243;][vc_row_inner limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; width=&#8221;1\/2&#8243;][vc_column_text]<strong>Culture systems optimized for the amplification of many cell types:<\/strong><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"2\"><span style=\"font-weight: 400;\">Adherent mammalian cells or cells in suspension <\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"2\">CHO, HEK293, HeLa, Cos, Caco-2, cell line supplied by the customer,&#8230;<\/li>\n<li style=\"font-weight: 400;\" aria-level=\"2\"><span style=\"font-weight: 400;\">Prokaryotic cells<\/span><\/li>\n<\/ul>\n<p><strong>Units dedicated to the production of eukaryotic cells (dedicated laboratories, clean rooms and controlled atmosphere, etc.)<\/strong><\/p>\n<p><strong>Production capacities allowing to supply pilot batches and also large-scale production batches (bulk):<\/strong><\/p>\n<ul>\n<li><span style=\"font-weight: 400;\">Supply from a<\/span><b> few millilitres up to several hundred litres of supernatant per batch<\/b><\/li>\n<li>Supply from a<b> few million to several billion cells per batch<\/b><\/li>\n<li style=\"list-style-type: none;\"><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; width=&#8221;1\/2&#8243;][vc_column_text]<strong>Supply of cells in the format suitable for the targeted applications:<\/strong><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"2\"><span style=\"font-weight: 400;\">Cryovials<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"2\"><span style=\"font-weight: 400;\">Culture plates (96 wells, 24 wells, 12 wells, 6 wells,&#8230;.)<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"2\"><span style=\"font-weight: 400;\">Culture boxes of different volumes, bottles\u2026.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"2\"><span style=\"font-weight: 400;\">Other custom packaging<\/span><\/li>\n<\/ul>\n<p><strong>At the end of the project, RD-Biotech provides express transport adapted to the cell model and packaging, the cells are accompanied by the production report and the quality controls carried out:<\/strong><\/p>\n<ul>\n<li>Mycoplasma control<\/li>\n<li>Cell viability test<\/li>\n<li>Stability control<\/li>\n<li>Sterility control<\/li>\n<li>Quantification of molecules<\/li>\n<li>Other controls on request<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;7&#8243; mobile_width=&#8221;7&#8243; css_animation=&#8221;zoom-out&#8221; width=&#8221;1\/1&#8243;][vc_gallery el_id=&#8221;gallery-197788&#8243; type=&#8221;carousel&#8221; medias=&#8221;89089,89093,89091&#8243; carousel_lg=&#8221;3&#8243; carousel_md=&#8221;1&#8243; carousel_sm=&#8221;1&#8243; thumb_size=&#8221;four-three&#8221; gutter_size=&#8221;3&#8243; carousel_height=&#8221;equal&#8221; carousel_interval=&#8221;3000&#8243; carousel_navspeed=&#8221;400&#8243; carousel_overflow=&#8221;yes&#8221; carousel_autoh=&#8221;yes&#8221; stage_padding=&#8221;0&#8243; single_overlay_opacity=&#8221;50&#8243; single_padding=&#8221;2&#8243;][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; back_color=&#8221;color-lxmt&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; animation_speed=&#8221;1000&#8243; width=&#8221;1\/1&#8243;]\n\t\t<div id=\"tdc_summary_544385\" class=\"tdc_summary text-lead wpb_content_element \">\n\n\t\t\t<div class=\"tdc_summary_title\">\n\t\t\t\t<a href=\"#\" class=\"summaryAnchor\">Summary<i class=\"fa fa-chevron-down close\" aria-hidden=\"true\"><\/i><\/a>\n\t\t\t<\/div>\n\n\t\t<\/div>\n\t\t[\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column width=&#8221;1\/3&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/3&#8243;][vc_custom_heading text_size=&#8221;h1&#8243; text_weight=&#8221;200&#8243; el_class=&#8221;picto_after_right&#8221;]Bioproduction[\/vc_custom_heading][\/vc_column][vc_column width=&#8221;1\/3&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;681043&#8243; row_name=&#8221;Production&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-193705&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/4&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243;]Supernatant production[\/vc_custom_heading][vc_column_text]This service includes the amplification of the cells and the supply of the culture supernatant: supply from a few millilitres up to several hundred litres of supernatant per batch.[\/vc_column_text][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;6&#8243; back_image=&#8221;89095&#8243; back_position=&#8221;center top&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/4&#8243; mobile_height=&#8221;280&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-109923&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/4&#8243;][vc_custom_heading text_color=&#8221;color-xsdn&#8221; heading_semantic=&#8221;h3&#8243;]Production of cell pellets[\/vc_custom_heading][vc_column_text]This service includes the amplification of cells and the supply of cell pellets: supply from a few million to several billion cells per batch.[\/vc_column_text][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_image=&#8221;89376&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/4&#8243; mobile_height=&#8221;280&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;126770&#8243; row_name=&#8221;Cell banks&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-142237&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243;]Cell banks[\/vc_custom_heading][vc_column_text]This service includes the constitution of cell banks: working banks and master banks, and can include the secure storage of these in liquid nitrogen in a controlled area.<\/p>\n<ul>\n<li>Reception of cells and mycoplasma control<\/li>\n<li>Cell amplification<\/li>\n<li>Packaging in 1 ml ampoules (1 to 5 million cells \/ ampoules)<\/li>\n<li>Quality controls after thawing<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;6&#8243; back_image=&#8221;88805&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/2&#8243; mobile_height=&#8221;280&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;679520&#8243; row_name=&#8221;Ready-to-use cells&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;6&#8243; back_image=&#8221;88808&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243; mobile_height=&#8221;280&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; shadow=&#8221;std&#8221; width=&#8221;1\/2&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243;]Ready-to-use cells[\/vc_custom_heading][vc_column_text]This service includes the amplification and supply of ready-to-use cells, according to customer specifications.<\/p>\n<p>Some examples:[\/vc_column_text][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;1. Production of CACO-2 cells&#8221; tab_id=&#8221;d21d5937-3bb0-21617960985185162376787651716238331504211623833317545162383366908816238337929571623834603545&#8243; slug=&#8221;caco-2-cells&#8221;][vc_column_text]<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">These cells are cultivated on a particular medium requiring perfect mastery of the amplification and culture monitoring processes.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">These cells are supplied in 96-well plates on specific supports, for pharmacological applications.<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;2. Production of blood cells&#8221; tab_id=&#8221;6dad8f3c-7309-81617960985185162376787651716238331504211623833317545162383366908816238337929571623834603545&#8243; slug=&#8221;blood-cells&#8221;][vc_column_text](from peripheral blood, cord blood, etc.)<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Preparation of cells from blood samples: PBMC (Peripheral blood mononuclear cells), Monocytes, Lymphocytes, CMBM (Cord blood mononuclear cells)\u2026<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Cell sorting and preparation of specific cells (CD4 +, CD34 +\u2026)<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Packaging in tubes, flasks, etc.<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;3. Production of primary cells&#8221; tab_id=&#8221;1623833716180-2-816238337929571623834603545&#8243; slug=&#8221;primary-cells&#8221;][vc_column_text](skin cells, adipocytes, preadipocytes, keratinocytes, etc.)<\/p>\n<ul>\n<li>Adaptation of culture conditions while respecting the number of passages specific to each cell type.<\/li>\n<li>Culture monitoring and supply of cells in the desired format.<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;7&#8243; top_padding=&#8221;4&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column width=&#8221;1\/4&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243;][vc_custom_heading text_size=&#8221;h1&#8243; text_weight=&#8221;200&#8243; el_class=&#8221;picto_after_right&#8221;]Custom services[\/vc_custom_heading][\/vc_column][vc_column width=&#8221;1\/4&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;117653&#8243; row_name=&#8221;Development of cell lines&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-109923&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243;]Development of cell lines[\/vc_custom_heading][vc_column_text]RD-Biotech offers a personalized service for the development of a cell line adapted to your specifications and your applications: expression of recombinant proteins or monoclonal antibodies, functional analysis of genes, drug screening, etc.<\/p>\n<ul>\n<li>If necessary, feasibility study to determine the best expression system and to define the optimal conditions for your application<\/li>\n<li>Adaptation according to the complexity of the project: Example, co-expression of different units\u2026<\/li>\n<li>Optimization and control of the vector or vectors: Possibility of carrying out vector construction for the project<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; hover_fx=&#8221;outlined&#8221; border_width=&#8221;0&#8243; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fen%2Fmolecular-biology%2F|title:biologie-moleculaire&#8221;]Discover molecular biology[\/vc_button][vc_column_text]<\/p>\n<ul>\n<li>Choice of parental cell line: CHO cells, HEK293, your own line\u2026<\/li>\n<li>Mode of transfection: lipofection, electroporation, etc.<\/li>\n<li>Screening platform: ELISA, Western Blotting, FACS, Octet, PCR \/ RT-PCR\u2026<\/li>\n<li>Royalty-free lineage<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; hover_fx=&#8221;outlined&#8221; border_width=&#8221;0&#8243; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fwp-content%2Fuploads%2F2021%2F07%2FCustom-development-service-of-stable-pools-or-stable-cell-lines-scaled.jpg|target:_blank&#8221;]Custom development service of stable pools or stable cell lines[\/vc_button][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; hover_fx=&#8221;outlined&#8221; border_width=&#8221;0&#8243; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fwp-content%2Fuploads%2F2023%2F05%2FRD-Biotech-Cell-line-engineering-service.jpg|target:_blank&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;169792&#8243;]Find out more[\/vc_button][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;6&#8243; back_image=&#8221;99905&#8243; back_position=&#8221;center bottom&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/2&#8243; mobile_height=&#8221;280&#8243; uncode_shortcode_id=&#8221;369404&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;6&#8243; back_image=&#8221;88816&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243; mobile_height=&#8221;280&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; back_color=&#8221;color-gyho&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/2&#8243;][vc_column_text]Stages of cell line development:[\/vc_column_text][vc_empty_space empty_h=&#8221;1&#8243;][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Step 1 : construction of the expression vector encoding the protein of interest&#8221; tab_id=&#8221;d21d5937-3bb0-21617960985185162376787651716238331504211623833317545162383366908816238337929571623834696087&#8243; slug=&#8221;vecto expression&#8221;][vc_column_text]<span style=\"font-weight: 400;\">Note: the vector can also be provided by customer<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Synthesis of the nucleotide sequence of interest (if necessary)<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Subcloning in the expression vector optimized for the cellular system<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Single-strand sequencing control<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Amplification and quality controls<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Step 2 : cell amplification&#8221; tab_id=&#8221;6dad8f3c-7309-81617960985185162376787651716238331504211623833317545162383366908816238337929571623834696087&#8243; slug=&#8221;cell-amplification&#8221;][vc_column_text]<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">CHO, HEK293 cell lines\u2026 or cell lines supplied by the customer.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Production of the cell pool necessary for transfection, culture in the medium adapted to the cell model<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Step 3 : transfection and expression test&#8221; tab_id=&#8221;1623833716180-2-816238337929571623834696087&#8243; slug=&#8221;transfection-and-expression-test&#8221;][vc_column_text]<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Transfection of host cells with the expression vector<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Expression test for the protein of interest (FACS, PCR, &#8230;)<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Step 4 : generation of stable pools&#8221; tab_id=&#8221;1623834762791-3-7&#8243; slug=&#8221;generation-of-stable-pools&#8221;][vc_column_text]<\/p>\n<ul>\n<li><span style=\"font-weight: 400;\">Transfection of cells and stable insertion of the gene for the protein of interest<\/span><\/li>\n<li><span style=\"font-weight: 400;\">Selection (choice of the selection agent)<\/span><\/li>\n<li><span style=\"font-weight: 400;\">Generation and characterization of several stable pools: ELISA, Western blotting, Flow cytometry, PCR \/ RT-PCR\u2026<\/span><\/li>\n<li><span style=\"font-weight: 400;\">Selection of the best candidates: best producer, target expression, etc.<\/span><\/li>\n<li><span style=\"font-weight: 400;\">Amplification of candidates<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">RD-Biotech can provide <\/span><b>stable pools<\/b><span style=\"font-weight: 400;\"> after controls (homogeneity test, stability study, etc.) and constitution of cell banks, MCB (10 ampoules of 2 million cells per pool)<\/span><\/p>\n<p><span style=\"font-weight: 400;\">If the customer wishes a <\/span><b>stable cell line<\/b><span style=\"font-weight: 400;\">, RD-Biotech will continue the development process<\/span>[\/vc_column_text][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Step 5 : cloning by limiting dilution and generation of stable clones&#8221; tab_id=&#8221;1623834788160-4-10&#8243; slug=&#8221;cloning-by-limiting&#8221;][vc_column_text]<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Isolation and selection of the best clone (s): isolation of clones by limiting dilution, screening by ELISA, Western Blot, flow cytometry, FACS, Byte, PCR, etc.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Isolation and expansion of the selected clone (s)<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Characterization of the best clone (s): evaluation of productivity, western blotting test, stability test, etc.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Supply of a stable cell line: Constitution of an MCB master cell bank (10 ampoules of 2 million cells per clone)<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Providing of a full report<\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; hover_fx=&#8221;outlined&#8221; text_skin=&#8221;yes&#8221; border_width=&#8221;0&#8243; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fwp-content%2Fuploads%2F2021%2F07%2FSteps-and-deadlines-Custom-development-service-of-stable-pools-or-stable-cell-lines.png|target:_blank&#8221;]Steps and deadlines[\/vc_button][\/vc_accordion_tab][\/vc_accordion][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;664966&#8243; row_name=&#8221;Transient transfection platform&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-193705&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243;]Transient transfection platform[\/vc_custom_heading][vc_column_text]The transient transfection platform makes it possible to produce from a few micrograms to several hundred milligrams of recombinant antibodies<span style=\"font-weight: 400;\"> or recombinant proteins<\/span><span style=\"font-weight: 400;\">\u00a0in about fifteen days.<\/span>[\/vc_column_text][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; display=&#8221;inline&#8221; top_margin=&#8221;yes&#8221; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fen%2Frecombinant-antibodies%2F%23bioproduction|title:Anticorps%20recombinants&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;477163&#8243;]Discover recombinant antibodies[\/vc_button][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; display=&#8221;inline&#8221; top_margin=&#8221;yes&#8221; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fen%2Frecombinant-proteins%2F|title:Prot%C3%A9ines%20recombinantes&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;194403&#8243;]Discover recombinant proteins[\/vc_button][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;6&#8243; back_image=&#8221;99903&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;1\/2&#8243; mobile_height=&#8221;280&#8243; back_size=&#8221;cover&#8221; uncode_shortcode_id=&#8221;902849&#8243;][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;5&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; 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