{"id":90481,"date":"2015-05-28T11:30:14","date_gmt":"2015-05-28T09:30:14","guid":{"rendered":"http:\/\/www.rd-biotech.com\/biologie-moleculaire\/"},"modified":"2025-04-24T09:23:37","modified_gmt":"2025-04-24T07:23:37","slug":"plasmid-dna","status":"publish","type":"page","link":"https:\/\/www.rd-biotech.com\/en\/plasmid-dna\/","title":{"rendered":"Plasmid DNA"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; style=&#8221;light&#8221; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;-4&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; shadow=&#8221;std&#8221; width=&#8221;1\/1&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;2&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; uncode_shortcode_id=&#8221;107504&#8243; limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;6&#8243; mobile_width=&#8221;7&#8243; width=&#8221;5\/6&#8243; uncode_shortcode_id=&#8221;115501&#8243;][vc_custom_heading heading_semantic=&#8221;p&#8221; text_size=&#8221;h4&#8243; uncode_shortcode_id=&#8221;624139&#8243; text_color_type=&#8221;uncode-solid&#8221; text_color_solid=&#8221;#cc93ed&#8221;]RD-Biotech\u2019s molecular biology platform offers a complete range of custom services.<\/p>\n<p>By contacting RD-Biotech, you will:<\/p>\n<ul>\n<li>save time while devoting yourself to the major areas of your high added value research,<\/li>\n<li>benefit from our scientific and technical expertise in molecular biology.<\/li>\n<\/ul>\n<p>[\/vc_custom_heading][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;2&#8243; mobile_width=&#8221;7&#8243; width=&#8221;1\/6&#8243; uncode_shortcode_id=&#8221;747647&#8243;][vc_single_image media=&#8221;99624&#8243; media_width_percent=&#8221;80&#8243; alignment=&#8221;center&#8221; uncode_shortcode_id=&#8221;204081&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; back_color=&#8221;color-lxmt&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; animation_speed=&#8221;1000&#8243; width=&#8221;1\/1&#8243;]\n\t\t<div id=\"tdc_summary_895610\" class=\"tdc_summary text-lead wpb_content_element \">\n\n\t\t\t<div class=\"tdc_summary_title\">\n\t\t\t\t<a href=\"#\" class=\"summaryAnchor\">Summary<i class=\"fa fa-chevron-down close\" aria-hidden=\"true\"><\/i><\/a>\n\t\t\t<\/div>\n\n\t\t<\/div>\n\t\t[\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;3&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;171556&#8243;][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;4&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;&#8221; position_horizontal=&#8221;left&#8221; gutter_size=&#8221;&#8221; overlay_alpha=&#8221;&#8221; shift_x=&#8221;&#8221; shift_y=&#8221;&#8221; shift_y_down=&#8221;&#8221; z_index=&#8221;&#8221; medium_width=&#8221;&#8221; mobile_width=&#8221;&#8221; css_animation=&#8221;left-t-right&#8221; width=&#8221;4\/12&#8243;][vc_icon position=&#8221;right&#8221; icon_image=&#8221;87906&#8243; media_size=&#8221;70&#8243; heading_semantic=&#8221;h2&#8243; text_size=&#8221;h1&#8243; text_weight=&#8221;200&#8243; title=&#8221;Bioproduction&#8221; el_class=&#8221;icon_box_chevrons icon_box_with_text&#8221;][\/vc_icon][\/vc_column_inner][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; width=&#8221;8\/12&#8243;][vc_column_text uncode_shortcode_id=&#8221;101597&#8243;]<strong>DNA PLASMID PRODUCTION<\/strong><\/p>\n<p>With more than <strong>1500 plasmids produced<\/strong> each year, RD-Biotech has developed unrivalled expertise in the <strong>production of plasmids<\/strong>, for research applications <span style=\"font-weight: 400;\">up to clinical studies.<\/span><\/p>\n<p>Whether you require 1 mg of DNA for an R&amp;D study or 500 mg for in vivo applications, our goal is to meet your demands, in terms of quantity, quality and deadline.<\/p>\n<p><span style=\"font-weight: 400;\">A GMP-Grade service: In order to complete its pDNA biomanufacturing platform, RD-Biotech now offers GMP Quality plasmid DNA, an essential raw material for new therapies: mRNA\/DNA based therapies, Cell therapies, virus-based therapies, \u2026<\/span>[\/vc_column_text][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:%2Fen%2Fgmp-plasmid-production-facilities%2F|target:_blank&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;157228&#8243;]DISCOVER[\/vc_button][\/vc_column_inner][\/vc_row_inner][vc_single_image media=&#8221;102086&#8243; media_width_percent=&#8221;75&#8243; alignment=&#8221;center&#8221; uncode_shortcode_id=&#8221;169700&#8243;][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fwp-content%2Fuploads%2F2023%2F11%2FRD-Biotech-pDNA-biomanufacturing-service-2023.pdf|target:_blank&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;183116&#8243;]FIND OUT MORE[\/vc_button][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;100&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; auto_height_device=&#8221;yes&#8221; style=&#8221;inherited&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;173468&#8243; row_name=&#8221;\u201cEndofree\u201d Research &amp; Development grade plasmid production&#8221;][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;4&#8243; back_color=&#8221;color-xsdn&#8221; back_image=&#8221;99885&#8243; back_position=&#8221;right bottom&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; animation_delay=&#8221;600&#8243; zoom_width=&#8221;0&#8243; zoom_height=&#8221;0&#8243; width=&#8221;5\/12&#8243; mobile_height=&#8221;280&#8243; uncode_shortcode_id=&#8221;200200&#8243; back_color_type=&#8221;uncode-palette&#8221;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-109923&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; animation_delay=&#8221;600&#8243; width=&#8221;7\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; uncode_shortcode_id=&#8221;821534&#8243;]\u201cEndofree\u201d Research &amp; Development grade plasmid production[\/vc_custom_heading][vc_custom_heading heading_semantic=&#8221;h4&#8243; text_size=&#8221;h4&#8243; uncode_shortcode_id=&#8221;117153&#8243;]<em>Plasmid DNA for your R&amp;D and preclinical applications<\/em>[\/vc_custom_heading][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Presentation&#8221; tab_id=&#8221;d21d5937-3bb0-216179609851851623767876517&#8243; slug=&#8221;presentation-bio-mol&#8221;][vc_row_inner limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; style=&#8221;dark&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; width=&#8221;1\/1&#8243;][vc_column_text uncode_shortcode_id=&#8221;619782&#8243;]<\/p>\n<p style=\"text-align: left;\"><strong>RD-Biotech is capable of supplying batches of 10 mg in 3 days, batches of 100 mg in only 6 days!<\/strong><\/p>\n<ul>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">\u201cEndofree\u201d quality: we guarantee a level of residual endotoxins of less than 0.1 EU \/ \u00b5g of DNA<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">High production capacity: from a few milligrams up to several grammes per plasmid. QUANTITY GUARANTEED!<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Very short deadlines<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Production of plasmids from DNA, bacterial spreads or glycerol stocks<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Pre-production controls according to the reference restriction profile: selection of the clone to be amplified, analysis of the restriction profile, quantification, etc.<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Final quality controls included, without additional cost: Quantification by spectrophotometry \u2013 Control by enzymatic digestion.<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">&gt;90% super-coiled DNA<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Homogeneity of batches<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Possibility of secure storage for recurring plasmid productions<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Adaptation of the process in case of low copy plasmids or recombinations.<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Other related services&#8221; tab_id=&#8221;6dad8f3c-7309-816179609851851623767876517&#8243; slug=&#8221;services-lies-bio-mol&#8221;][vc_row_inner limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; style=&#8221;dark&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243;][vc_column_text uncode_shortcode_id=&#8221;374574&#8243;]<\/p>\n<ul>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Bacterial transformation<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Adjustment to the desired DNA concentration<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Aliquoting<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Sequencing control (single or double strand, insert or entire plasmid)<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Measurement of the supercoiled rate<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Determination of bacterial endotoxins (LAL \/ Limulus Amebocyte Lysate test)<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Sterility test<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Sequencing<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Others: <a href=\"https:\/\/www.rd-biotech.com\/en\/contact\/\">contact us<\/a><\/span><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Examples of applications&#8221; tab_id=&#8221;9346230d-a835-916179609851851623767876517&#8243; slug=&#8221;exemple-application-bio-mol&#8221;][vc_row_inner limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; style=&#8221;dark&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243;][vc_column_text uncode_shortcode_id=&#8221;190761&#8243;]<\/p>\n<ul>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Transfection \/ Co-transfection in mammalian cells<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Production of recombinant proteins and recombinant antibodies<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\"><em>In vivo<\/em> studies (injection in animals)<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Preclinical studies<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Toxicology studies<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Research &amp; Development applications<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_accordion_tab][\/vc_accordion][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fen%2Fanalytical-services%2F|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;198239&#8243;]DISCOVER ANALYTICAL SERVICES[\/vc_button][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;100&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; style=&#8221;inherited&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;363407&#8243; row_name=&#8221;High Quality Grade (\u201cGMP-like\u201d grade) plasmid production&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; back_color=&#8221;color-gyho&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; animation_delay=&#8221;600&#8243; zoom_width=&#8221;0&#8243; zoom_height=&#8221;0&#8243; width=&#8221;7\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; uncode_shortcode_id=&#8221;169871&#8243;]High Quality Grade (\u201cGMP-like\u201d grade) plasmid production[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;642670&#8243;]<\/p>\n<p style=\"text-align: left;\">Production of superior quality plasmids, suitable for clinical applications, as raw materials in cell and gene therapy programs (AAV, lentivirus, retrovirus, therapeutic mRNA \u2026)<\/p>\n<ul>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Complete traceability and specific documentation for each project<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Constitution of a bacterial cell bank for each plasmid<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Storage of the bacterial bank in a secure area<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Each plasmid is produced independently (1 plasmid \/ series) in a dedicated room.<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Large-scale culture performed without antibiotics<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">The production process is carried out \u201canimal free\u201d \u2013 without substances of animal origin<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Additional steps are applied to guarantee maximum product purity (quality standards compatible with raw materials for GMP grade production)<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Quality controls are adapted to the custom specifications and to the regulation (% supercoiled DNA, bacterial DNA, residual host proteins, bacterial RNA, Endotoxins\u2026 <a href=\"http:\/\/www.rd-biotech.com\/wp-content\/uploads\/2023\/12\/RD-Biotech-pDNA-biomanufacturing-QC.png\" target=\"_blank\" rel=\"noopener\">list of QCs<\/a>)<\/li>\n<li aria-level=\"1\">Option : Supply of linearized plasmid DNA, for IVT applications (mRNA preparations)<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; hover_fx=&#8221;outlined&#8221; border_width=&#8221;0&#8243; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fen%2Fanalytical-services%2F|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;778871&#8243;]DISCOVER ANALYTICAL SERVICES[\/vc_button][vc_single_image media=&#8221;91282&#8243; media_lightbox=&#8221;yes&#8221; media_width_percent=&#8221;100&#8243; advanced=&#8221;yes&#8221; media_overlay_opacity=&#8221;50&#8243; media_padding=&#8221;2&#8243; lbox_skin=&#8221;white&#8221; uncode_shortcode_id=&#8221;482940&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;4&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; back_image=&#8221;99897&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; animation_delay=&#8221;600&#8243; width=&#8221;5\/12&#8243; mobile_height=&#8221;280&#8243; uncode_shortcode_id=&#8221;271170&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;100&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; style=&#8221;inherited&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;612997&#8243; row_name=&#8221;GMP grade plasmid production&#8221;][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;4&#8243; back_color=&#8221;color-xsdn&#8221; back_image=&#8221;99893&#8243; back_position=&#8221;right bottom&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; animation_delay=&#8221;600&#8243; zoom_width=&#8221;0&#8243; zoom_height=&#8221;0&#8243; width=&#8221;5\/12&#8243; mobile_height=&#8221;280&#8243; uncode_shortcode_id=&#8221;148561&#8243; back_color_type=&#8221;uncode-palette&#8221;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-193705&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; animation_delay=&#8221;600&#8243; width=&#8221;7\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; uncode_shortcode_id=&#8221;812400&#8243;]GMP grade plasmid production[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;791161&#8243;]Opening of our GMP grade pDNA production facilities in Q3 2023, to complement our current DNA plasmid production capacities in R&amp;D and \u201cHigh Quality\u201d grades, to meet growing new cell and gene therapy market demands.<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\">More than 1,200 m2 of GMP grade laboratories<\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\">Several independent production suites<\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\">GMP scale: up to 20 g of highly purified pDNA<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; display=&#8221;inline&#8221; top_margin=&#8221;yes&#8221; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fwp-content%2Fuploads%2F2024%2F01%2FRD-Biotech-pDNA-biomanufacturing-service-Overview.png&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;218811&#8243;]FIND OUT MORE[\/vc_button][vc_button button_color=&#8221;color-xsdn&#8221; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; display=&#8221;inline&#8221; top_margin=&#8221;yes&#8221; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fen%2Fgmp-plasmid-production-facilities%2F&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;161466&#8243;]GMP grade service[\/vc_button][\/vc_column][\/vc_row][vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;7&#8243; top_padding=&#8221;4&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243;][vc_column width=&#8221;1\/4&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; width=&#8221;1\/2&#8243;][vc_custom_heading text_size=&#8221;h1&#8243; text_weight=&#8221;200&#8243; el_class=&#8221;picto_after_right&#8221; uncode_shortcode_id=&#8221;145780&#8243;]Contract services[\/vc_custom_heading][\/vc_column][vc_column width=&#8221;1\/4&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;100&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; auto_height_device=&#8221;yes&#8221; style=&#8221;inherited&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;766130&#8243; row_name=&#8221;Vector cloning&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; back_color=&#8221;color-lxmt&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; animation_delay=&#8221;600&#8243; width=&#8221;2\/3&#8243; uncode_shortcode_id=&#8221;127792&#8243; back_color_type=&#8221;uncode-palette&#8221;][vc_column_text uncode_shortcode_id=&#8221;134369&#8243;]<span style=\"font-weight: 400;\">Dedicated to excellence in molecular biology, RD-Biotech provides cutting-edge solutions precisely tailored to meet the requirements of customers. Our services encompass various expertise areas, ranging from the construction of plasmid vectors \u2014 crucial elements in DNA and RNA-based therapies or recombinant protein expression vectors \u2014 to high quality RNA or DNA preparation, or even monoclonal antibody sequencing for optimal clone preservation and recombinants expression, \u2026<\/span><\/p>\n<p><b>Why choose our services:<\/b><\/p>\n<ol>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Customized Service:<\/b><span style=\"font-weight: 400;\"> tailored to your specific needs, ensuring a personalized approach for each project.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Cutting-edge Technology:<\/b><span style=\"font-weight: 400;\"> continually investment to guarantee high-quality results.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Expert Team:<\/b> a team of experienced scientists to guide you at every stage, from understanding your needs to delivering the final product and analysis report.<\/li>\n<\/ol>\n<p>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/3&#8243;][vc_single_image media=&#8221;102097&#8243; media_width_percent=&#8221;100&#8243; uncode_shortcode_id=&#8221;190351&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;100&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; auto_height_device=&#8221;yes&#8221; style=&#8221;inherited&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;766130&#8243; row_name=&#8221;Vector cloning&#8221;][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;4&#8243; back_color=&#8221;color-xsdn&#8221; back_image=&#8221;99895&#8243; back_position=&#8221;center center&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; animation_delay=&#8221;600&#8243; zoom_width=&#8221;0&#8243; zoom_height=&#8221;0&#8243; width=&#8221;5\/12&#8243; mobile_height=&#8221;280&#8243; uncode_shortcode_id=&#8221;930209&#8243; back_color_type=&#8221;uncode-palette&#8221;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; back_color=&#8221;color-109607&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; animation_delay=&#8221;600&#8243; width=&#8221;7\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; uncode_shortcode_id=&#8221;115352&#8243;]Vector cloning[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;102630&#8243;]<\/p>\n<p style=\"text-align: left;\">RD-Biotech can take charge of the construction or modification of your DNA vectors, whatever their application, through a personalized service in order to meet the exact needs of our customers.<\/p>\n<p>[\/vc_column_text][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Custom cloning service&#8221; tab_id=&#8221;d21d5937-3bb0-2161796098518516237678765171623833150421&#8243; slug=&#8221;clonage-sur-mesure&#8221;][vc_row_inner limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; width=&#8221;1\/1&#8243;][vc_column_text uncode_shortcode_id=&#8221;968809&#8243;]<\/p>\n<p style=\"text-align: left;\">Our team of molecular biology specialists can work with you to define the best cloning strategy according to your specifications:<\/p>\n<ul>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\"><em>In silico<\/em> study<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Choice and design of the nucleic acid sequence of interest<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Optimization of the sequence if required<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Sequence synthesis (PCR, gene synthesis)<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Choice of cloning vector (tag addition, promoter sequences, insertion of specific cleavage sites, co-expression \/ or not \u2026)<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Choice of cloning technology: Homologous recombination, Enzymatic restriction \/ ligation, Mutagenesis, Golden Gate&#8230;<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Starting material&#8221; tab_id=&#8221;6dad8f3c-7309-8161796098518516237678765171623833150421&#8243; slug=&#8221;materiel-depart&#8221;][vc_row_inner limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243;][vc_column_text uncode_shortcode_id=&#8221;189197&#8243;]<\/p>\n<ul>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Nucleotide or protein sequence (with or without optimization)<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Insert in a shuttle vector<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;A complete service&#8221; tab_id=&#8221;9346230d-a835-9161796098518516237678765171623833150421&#8243; slug=&#8221;service-complet&#8221;][vc_row_inner limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243;][vc_column_text uncode_shortcode_id=&#8221;708230&#8243;]<\/p>\n<ul>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Cloning according to an optimized strategy<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Plasmid production: from a few \u00b5g of DNA up to large scale production<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Quality controls:\n<ul>\n<li>Quantification by spectrophotometry<\/li>\n<li>Control of restriction profile by agarose gel electrophoresis<\/li>\n<li>Sequencing (full insert)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Personalized service&#8221; tab_id=&#8221;1623833213260-3-6&#8243; slug=&#8221;suivi-personnalise&#8221;][vc_column_text uncode_shortcode_id=&#8221;199274&#8243;]A dedicated project manager for each project, in order to ensure constant monitoring and to provide responsiveness as to the progress of the cloning project.<\/p>\n<p>Each project is made up of successive stages, each stage carried out can be validated by the customer before moving on to the next stage (GO \/ NO GO), for more <strong>flexibility and security<\/strong>.<\/p>\n<p><strong>Confidentiality guaranteed.<\/strong>[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion][vc_single_image media=&#8221;91284&#8243; media_lightbox=&#8221;yes&#8221; media_width_percent=&#8221;100&#8243; advanced=&#8221;yes&#8221; media_overlay_opacity=&#8221;50&#8243; media_padding=&#8221;2&#8243; lbox_skin=&#8221;white&#8221; uncode_shortcode_id=&#8221;168071&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;100&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; style=&#8221;inherited&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;180462&#8243; row_name=&#8221;Sequencing of monoclonal antibodies&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; animation_delay=&#8221;600&#8243; shadow=&#8221;std&#8221; zoom_width=&#8221;0&#8243; zoom_height=&#8221;0&#8243; width=&#8221;7\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; el_id=&#8221;sequencage-anticorps-monoclonaux-bio-mol&#8221; uncode_shortcode_id=&#8221;195790&#8243;]Sequencing of monoclonal antibodies[\/vc_custom_heading][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; display=&#8221;inline&#8221; top_margin=&#8221;yes&#8221; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fen%2Fmonoclonal-antibodies%2F|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;161202&#8243;]DISCOVER MONOCLONAL ANTOBODIES[\/vc_button][vc_button button_color=&#8221;color-109923&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; display=&#8221;inline&#8221; top_margin=&#8221;yes&#8221; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fen%2Frecombinant-antibodies%2F|title:Anticorps%20recombinants|target:_blank|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;971801&#8243;]DISCOVER RECOMBINANT ANTIBODIES[\/vc_button][vc_column_text uncode_shortcode_id=&#8221;176852&#8243;]<\/p>\n<p style=\"text-align: left;\">RD-Biotech offers a <strong>fast and reliable sequencing service<\/strong> of your monoclonal antibodies in order to:<\/p>\n<ul>\n<li style=\"text-align: left;\">The safeguard and securing of the sequence of your antibody of interest<\/li>\n<li style=\"text-align: left;\">Determination of the nucleotide sequences of the heavy and light variable chains in order to produce the antibody in <strong>recombinant form<\/strong><\/li>\n<li style=\"text-align: left;\">Starting material: hybridoma cells (in culture or frozen pellet) or dry cell pellet<\/li>\n<li style=\"text-align: left;\">Sequencing of variable regions of heavy and light chains or complete sequencing of the antibody<\/li>\n<li style=\"text-align: left;\">Results in less than 2 weeks<\/li>\n<li style=\"text-align: left;\"><strong>Price discounted<\/strong> as part of a complete project: Generation of hybridomas \u2013 Construction of the vector and development of recombinants (Backbone IgG murine, human, guinea pig, rat \u2026 or other<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_button button_color=&#8221;color-193705&#8243; radius=&#8221;btn-circle&#8221; border_width=&#8221;0&#8243; link=&#8221;url:http%3A%2F%2Fwww.rd-biotech.com%2Fen%2Fcontact%2F|&#8221; button_color_type=&#8221;uncode-palette&#8221; uncode_shortcode_id=&#8221;544518&#8243;]CONTACT US[\/vc_button][vc_single_image media=&#8221;91286&#8243; media_lightbox=&#8221;yes&#8221; media_width_percent=&#8221;100&#8243; advanced=&#8221;yes&#8221; media_overlay_opacity=&#8221;50&#8243; media_padding=&#8221;2&#8243; lbox_skin=&#8221;white&#8221; uncode_shortcode_id=&#8221;138682&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;4&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; back_image=&#8221;99864&#8243; back_repeat=&#8221;no-repeat&#8221; back_position=&#8221;center center&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; animation_delay=&#8221;600&#8243; width=&#8221;5\/12&#8243; mobile_height=&#8221;280&#8243; uncode_shortcode_id=&#8221;147916&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;100&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; style=&#8221;inherited&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;171860&#8243; row_name=&#8221;Nucleic acid extraction&#8221;][vc_column column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;2&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-lxmt&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; animation_delay=&#8221;600&#8243; zoom_width=&#8221;0&#8243; zoom_height=&#8221;0&#8243; width=&#8221;5\/12&#8243; mobile_height=&#8221;280&#8243;][vc_single_image media=&#8221;91288&#8243; media_lightbox=&#8221;yes&#8221; media_width_percent=&#8221;100&#8243; advanced=&#8221;yes&#8221; media_overlay_opacity=&#8221;50&#8243; media_padding=&#8221;2&#8243; lbox_skin=&#8221;white&#8221; uncode_shortcode_id=&#8221;123636&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-142237&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; animation_delay=&#8221;600&#8243; width=&#8221;7\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; el_id=&#8221;extraction-acides-nucleiques&#8221; uncode_shortcode_id=&#8221;159075&#8243;]Nucleic acid extraction[\/vc_custom_heading][vc_custom_heading heading_semantic=&#8221;h4&#8243; text_size=&#8221;h4&#8243; uncode_shortcode_id=&#8221;887666&#8243;]<em>Preparation of RNA and genomic DNA<\/em>[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;326725&#8243;]Preparation and purification of RNA and genomic DNA from different biological samples (blood, cell pellets, biopsies, etc.) for research applications or preclinical studies.<\/p>\n<p>The procedure for extraction, purification and control of total RNA and DNA is optimized according to the starting biological material.<\/p>\n<ul style=\"text-align: left;\">\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Extraction and purification platform dedicated to the preparation of total RNA and DNA<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Large production capacity: preparation by series of 50 to 200 samples<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Possibility of preserving RNA and DNA preparations (secure guarding) for later uses (Reverse transcription, PCR, Cloning, etc.)<\/li>\n<\/ul>\n<p style=\"text-align: left;\"><strong>Quality controls:<\/strong><\/p>\n<ul>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">RNA and DNA quantification by spectrophotometry<br \/>\nControls of RNA integrity on chips<br \/>\nControls of DNA and RNA on agarose gel<br \/>\nOther controls \u2013 (<a href=\"https:\/\/rd-biotech.tetedecom.pro\/en\/contact\/\" target=\"_blank\" rel=\"noopener\">contact us<\/a>)<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;100&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; style=&#8221;inherited&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;175745&#8243; row_name=&#8221;Nucleic acid amplification: RT-PCR qPCR&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-109923&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; animation_delay=&#8221;600&#8243; zoom_width=&#8221;0&#8243; zoom_height=&#8221;0&#8243; width=&#8221;7\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; el_id=&#8221;amplification-acides-nucleiques&#8221; uncode_shortcode_id=&#8221;142533&#8243;]Nucleic acid amplification: RT-PCR qPCR[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;149218&#8243;]<\/p>\n<p style=\"text-align: left;\">RD-Biotech can respond to various issues related to the quantification of genes, the study of gene expression, the analysis of the expression of different variants of a gene, the identification of specific sequences &#8230;<\/p>\n<p>[\/vc_column_text][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Classic PCR and RT-PCR Platform&#8221; tab_id=&#8221;d21d5937-3bb0-21617960985185162376787651716238331504211623833317545&#8243; slug=&#8221;plate-forme-de-pcr-et-rt-pcr-classique&#8221;][vc_row_inner limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; style=&#8221;dark&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; width=&#8221;1\/1&#8243;][vc_column_text uncode_shortcode_id=&#8221;521189&#8243;]<\/p>\n<ul>\n<li>Development of operating conditions or application of a validated procedure<\/li>\n<li>Amplifications by PCR<\/li>\n<li>PCR products control by sequencing \u2013 if requested<\/li>\n<li>Analysis of results and delivery of a complete report<\/li>\n<li>Possibility of preparing samples before analysis<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;2&#8243; title=&#8221;Real-time PCR Platform&#8221; tab_id=&#8221;6dad8f3c-7309-81617960985185162376787651716238331504211623833317545&#8243; slug=&#8221;plate-forme-de-pcr-en-temps-r\u00e9el&#8221;][vc_row_inner limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; style=&#8221;dark&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243;][vc_column_text uncode_shortcode_id=&#8221;507021&#8243;]<\/p>\n<ul>\n<li>Development of operating conditions or application of a validated procedure<\/li>\n<li>Absolute quantification and relative quantification<\/li>\n<li>SyberGreen\u00ae or TaqMan\u00ae procedure<\/li>\n<li>Analysis of results and delivery of a complete report<\/li>\n<li>Possibility of preparing samples before analysis<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_accordion_tab][\/vc_accordion][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;4&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; back_image=&#8221;88291&#8243; back_attachment=&#8221;inherit&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; animation_delay=&#8221;600&#8243; width=&#8221;5\/12&#8243; back_size=&#8221;contain&#8221; mobile_height=&#8221;280&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;100&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; style=&#8221;inherited&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;113449&#8243; row_name=&#8221;Cell line validation \/ Genotyping&#8221;][vc_column column_width_percent=&#8221;100&#8243; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;4&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-193705&#8243; back_image=&#8221;88299&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; animation_delay=&#8221;600&#8243; zoom_width=&#8221;0&#8243; zoom_height=&#8221;0&#8243; width=&#8221;5\/12&#8243; mobile_height=&#8221;280&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; style=&#8221;dark&#8221; back_color=&#8221;color-193705&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;right-t-left&#8221; animation_delay=&#8221;600&#8243; width=&#8221;7\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; uncode_shortcode_id=&#8221;204744&#8243;]Cell line validation \/ Genotyping[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;153438&#8243;]<\/p>\n<ul>\n<li style=\"text-align: left;\">Control of transgenic sequences<\/li>\n<li style=\"text-align: left;\">Measurement of the expression levels of target genes by <strong>qPCR<\/strong><\/li>\n<li style=\"text-align: left;\">SyberGreen\u00ae or TaqMan\u00ae procedure<\/li>\n<li style=\"text-align: left;\">Absolute quantification and relative quantification<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_column_text uncode_shortcode_id=&#8221;167240&#8243;]<\/p>\n<p style=\"text-align: left;\"><strong>A service adapted to your needs:<\/strong><\/p>\n<ul>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Preparation of genetic material<\/span><\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Development of operating conditions or application of validated procedure<\/li>\n<li style=\"font-weight: 400; text-align: left;\" aria-level=\"1\">Detailed analysis report<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;3&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;2&#8243; overlay_alpha=&#8221;100&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;3&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; style=&#8221;inherited&#8221; el_class=&#8221;no-padding-left-right-mobile&#8221; uncode_shortcode_id=&#8221;437412&#8243; row_name=&#8221;RNA preparation by in vitro transcription&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;3&#8243; back_color=&#8221;color-gyho&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;left-t-right&#8221; animation_delay=&#8221;600&#8243; zoom_width=&#8221;0&#8243; zoom_height=&#8221;0&#8243; width=&#8221;7\/12&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; uncode_shortcode_id=&#8221;868009&#8243;]RNA preparation by <em>in vitro<\/em> transcription[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;105602&#8243;]<em>In vitro<\/em> transcription platform <a href=\"http:\/\/www.rd-biotech.com\/wp-content\/uploads\/2023\/12\/RD-Biotech-RNA-preparation-service-2023.pdf\" target=\"_blank\" rel=\"noopener\">for the generation of RNAs<\/a>, from your plasmids or your PCR products<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\">Sense or antisense transcripts<\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\">Labelled or unlabelled transcripts<\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\">Capped and \/ or poly-adenylated transcripts<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_accordion history=&#8221;yes&#8221; active_tab=&#8221;0&#8243;][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;1&#8243; title=&#8221;Linearisation of purified plasmid&#8221; tab_id=&#8221;d21d5937-3bb0-216179609851851623767876517162383315042116238333175451623833440059&#8243; slug=&#8221;produit-pcr&#8221;][vc_row_inner limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; width=&#8221;1\/1&#8243;][vc_column_text uncode_shortcode_id=&#8221;660204&#8243;]<\/p>\n<ul>\n<li>Enzymatic digestion<\/li>\n<li>Pilote and large scale linearisation<\/li>\n<li>Purification and quality controls<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_accordion_tab][vc_accordion_tab gutter_size=&#8221;2&#8243; column_padding=&#8221;1&#8243; title=&#8221;RNA preparation by in vitro transcription&#8221; tab_id=&#8221;6dad8f3c-7309-816179609851851623767876517162383315042116238333175451623833440059&#8243; slug=&#8221;adn-plasmique&#8221;][vc_row_inner limit_content=&#8221;&#8221;][vc_column_inner column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; width=&#8221;1\/1&#8243;][vc_column_text el_class=&#8221;v-tabs-larger&#8221; uncode_shortcode_id=&#8221;151711&#8243;]<\/p>\n<ul>\n<li>Capping<\/li>\n<li>Modified nucleotides incorporation<\/li>\n<li>Poly-adenylation \u2013 polyA tail or post-transcriptionnal process (enzymatic)<\/li>\n<li>Quantity: from some \u00b5g up to <strong>batches of 5 -10 mg<\/strong><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_accordion_tab][\/vc_accordion][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; 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